PMID: 8993351Oct 25, 1996Paper

Posttranslational modifications of meningococcal pili. Identification of a common trisaccharide substitution on variant pilins of strain C311

Annals of the New York Academy of Sciences
Mumtaz VirjiE R Moxon

Abstract

Neisseria meningitidis pili are filamentous protein structures that are essential adhesins in capsulate bacteria. Pili of adhesion variants of meningococcal strain C311 contain glycosyl residues on pilin (PilE), their major structural subunit. Recent studies have shown that a novel O-linked trisaccharide substituent, not previously found as a constituent of glycoproteins, is present within a peptide spanning amino acid residues 50 to 73 of the PilE molecule. The structure was shown to be Gal beta 1-4 Gal alpha 1-3 diacetamidotrideoxyhexose which is directly attached to pilin. Pilins derived from galactose epimerase (galE) mutants lack the digalactosyl moiety, but retain the diacetamidotrideoxyhexose substitution. These studies confirm our previous observations that meningococcal pili are glycosylated and provide the first structural evidence for the presence of covalently linked carbohydrate on pili. We have identified a completely novel protein/carbohydrate linkage on a multimeric protein that is an essential virulence determinant in N. meningitidis.

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Citations

Jan 1, 2013·Biology·Miao-Chiu Hung, Myron Christodoulides
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Aug 11, 2021·Biochemical Society Transactions·Lok ManStuart J Cordwell

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