Posttranslational modifications of trichomonad tubulins; identification of multiple glutamylation sites

FEBS Letters
A SchneiderK Weber

Abstract

The alpha- and beta-tubulins present in cytoskeletons of Tritrichomonas mobilensis are extensively glutamylated. Automated sequencing and mass spectrometry of the carboxyterminal peptides identifies 4 glutamylation sites in alpha- and 2 sites in beta-tubulin. They are marked by asterisks in the terminal sequences GDE*E*E*E*DDG (alpha) and EGE*E*DEEAEA (beta). This is the first report that tubulin glutamylation can occur at multiple sites. Although T. mobilensis has four flagellae the tubulins lack polyglycylation. Thus glycylation is not necessary for formation or function of axonemal microtubules. Alpha-tubulin is completely acetylated at lysine 40 and shows no tyrosine cycle. Peptide sequences establish two distinct beta-tubulins.

References

Aug 4, 1975·Biochemical and Biophysical Research Communications·D Raybin, M Flavin
Oct 29, 1991·Biochemistry·L Paturle-LafanechèreD Job
Jan 5, 1990·Science·B EddéP Denoulet
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J E AlexanderA Frankfurter
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M LeDizet, G Piperno
May 8, 1995·FEBS Letters·M RüdigerK Weber
Jun 1, 1995·The Journal of Cell Biology·J GaertigM A Gorovsky
Mar 1, 1994·Molecular and Biochemical Parasitology·S K Katiyar, T D Edlind
Feb 1, 1993·The Journal of Cell Biology·K ErsfeldK Weber
Dec 1, 1993·Microbiological Reviews·T Cavalier-Smith
May 1, 1993·Molecular and Biochemical Parasitology·D D LeipeM L Sogin
Apr 26, 1996·The Journal of Biological Chemistry·J MaryJ M Schmitter
Jan 1, 1996·Cell Motility and the Cytoskeleton·P Delgado-ViscogliosiE Viscogliosi
Dec 1, 1996·Molecular Biology and Evolution·P J Keeling, W F Doolittle

❮ Previous
Next ❯

Citations

Mar 8, 2000·Cell Motility and the Cytoskeleton·H T Idriss
Nov 19, 2004·Parasitology Research·Uwe PlessmannKarl-Ferdinand Lechtreck
Feb 16, 2016·Cell·Max L Valenstein, Antonina Roll-Mecak
Oct 14, 2014·Seminars in Cell & Developmental Biology·Antonina Roll-Mecak
May 12, 2015·Cell·Christopher P GarnhamAntonina Roll-Mecak
Nov 5, 1999·Annual Review of Microbiology·K Gull
Feb 23, 2010·Biochemical and Biophysical Research Communications·Mai HasegawaShoji Odani
May 18, 2004·Cell Motility and the Cytoskeleton·Caterina MencarelliRomano Dallai
Nov 28, 2017·Scientific Reports·Isabelle DevambezLaurent Soustelle
Jan 22, 2008·Cell Motility and the Cytoskeleton·Henry D HoyleElizabeth C Raff
Jul 1, 2005·Cell Motility and the Cytoskeleton·Caterina MencarelliRomano Dallai
Aug 5, 2005·Cell Motility and the Cytoskeleton·Ellen M PopodiElizabeth C Raff
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Stefan Westermann, Klaus Weber
Jul 10, 2019·Nature Structural & Molecular Biology·Colby R SandateAntonina Roll-Mecak
Apr 20, 2002·Current Opinion in Infectious Diseases·Michael W. Lehker, John F. Alderete
Jun 4, 2017·Proceedings of the National Academy of Sciences of the United States of America·Christopher P GarnhamAntonina Roll-Mecak
Aug 5, 2020·Nature Structural & Molecular Biology·Kishore K MahalinganAntonina Roll-Mecak
Apr 5, 2000·The Journal of Biological Chemistry·C RegnardB Eddé
Jul 8, 2020·Developmental Cell·Antonina Roll-Mecak
Jul 25, 2021·International Journal of Molecular Sciences·Tatiana K RostovtsevaDavid P Hoogerheide
Dec 20, 2021·Seminars in Cell & Developmental Biology·Ewa Joachimiak, Dorota Wloga

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.