PMID: 2504694Sep 1, 1989Paper

Posttranslational regulatory system for nitrogenase activity in Azospirillum spp

Journal of Bacteriology
H A FuR H Burris

Abstract

The mechanism for "NH4+ switch-off/on" of nitrogenase activity in Azospirillum brasilense and A. lipoferum was investigated. A correlation was established between the in vivo regulation of nitrogenase activity by NH4Cl or glutamine and the reversible covalent modification of dinitrogenase reductase. Dinitrogenase reductase ADP-ribosyltransferase (DRAT) activity was detected in extracts of A. brasilense with NAD as the donor molecule. Dinitrogenase reductase-activating glycohydrolase (DRAG) activity was present in extracts of both A. brasilense and A. lipoferum. The DRAG activity in A. lipoferum was membrane associated, and it catalyzed the activation of inactive nitrogenase (by covalent modification of dinitrogenase reductase) from both A. lipoferum and Rhodospirillum rubrum. A region homologous to R. rubrum draT and draG was identified in the genomic DNA of A. brasilense as a 12-kilobase EcoRI fragment and in A. lipoferum as a 7-kilobase EcoRI fragment. It is concluded that a posttranslational regulatory system for nitrogenase activity is present in A. brasilense and A. lipoferum and that it operates via ADP-ribosylation of dinitrogenase reductase as it does in R. rubrum.

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Citations

Jul 15, 1997·FEMS Microbiology Letters·Y ZhangG P Roberts
Aug 22, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D P PotrichI S Schrank
Oct 24, 2009·The Journal of Microbiology·András PenyigeSándor Biró
Dec 23, 2011·Molecular Microbiology·Abhijit SarkarBarbara Reinhold-Hurek
Jun 11, 2015·PloS One·Robert L RobsonToby H Richardson
Sep 10, 2005·Applied and Environmental Microbiology·Giseli KlassenFábio O Pedrosa
Oct 24, 2001·Journal of Bacteriology·G KlassenF de O Pedrosa
Dec 1, 1995·Microbiological Reviews·M J Merrick, R A Edwards
Mar 1, 1993·Applied and Environmental Microbiology·J P ZehrD G Capone
May 18, 2011·BMC Microbiology·Fernando H Sant'annaIrene S Schrank

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