Posttransplant diastolic hypertension: associations with intragraft transforming growth factor-beta, endothelin, and renin transcription

Transplantation
A D KirkJ D Pirsch

Abstract

Diastolic hypertension after renal transplantation leads to significant chronic morbidity and mortality. Recently, calcineurin phosphatase inhibition by cyclosporine or tacrolimus has been postulated to lead to diastolic hypertension through the induction of transforming growth factor-beta (TGF-beta) and resultant endothelin-mediated renal arteriolar vasospasm. To investigate this hypothesis in humans, the allografts of 40 stable renal allograft recipients were biopsied 2 to 3 years after transplantation. Both cyclosporine and tacrolimus patients were included. Biopsies were divided and processed for histology and RNA extraction. RNA was then converted to cDNA and evaluated by semiquantitative polymerase chain reaction (actin-standardized, high-performance liquid chromatography-quantitated) for TGF-beta, endothelin, and renin transcription. Inflammatory cytokine gene transcription was also evaluated. Blood pressure was measured during the clinic check-in before biopsy. Variables were evaluated by Spearman rank correlation coefficient (rs) analysis. Diastolic hypertension was prevalent in the study population, with 40% of individuals having diastolic pressure >90 mmHg. TGF-beta and endothelin transcription were detected in 88% o...Continue Reading

References

Dec 21, 1989·The New England Journal of Medicine·B D Kahan
Mar 1, 1994·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J J Curtis
Jan 1, 1996·Journal of the American Society of Nephrology : JASN·B L KasiskeJ Z Ma

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Citations

Jan 21, 2004·Journal of Evaluation in Clinical Practice·W Adam Jurewicz, Andrew Miles
Feb 4, 1999·Hypertension·Y TakedaH Mabuchi
Dec 29, 1998·Current Opinion in Nephrology and Hypertension·P A Keown
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·A D Kirk
Sep 12, 2007·Clinical Transplantation·Suja NagarajanMinnie M Sarwal
Sep 8, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·C ChareandeeM S Simonson

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