Potassium-39 nuclear magnetic resonance observation of intracellular potassium without chemical shift reagents during metabolic inhibition in the isolated perfused rat heart

Circulation Research
S KukiY Kawashima

Abstract

The intracellular potassium content of perfused rat heart was measured by potassium-39 nuclear magnetic resonance (NMR) spectroscopy at 33 degrees C with an inversion recovery technique based on the fact that the spin-lattice relaxation time (T1) of the intracellular potassium (8.3 msec at 8.45 T) is much faster than that of the extracellular potassium (68 msec). Intracellular potassium decreased to 60.2 +/- 4.3% of the control level (mean +/- SEM, n = 6) at 40 minutes from the start of metabolic inhibition (2 mM cyanide, 0 mM glucose). Removal of cyanide restored intracellular potassium to 94.2 +/- 3.9% at 30 minutes from the restart of oxidative metabolism. The cumulative potassium loss was determined from the flow rate and potassium concentration of the coronary effluent, which reached 139 +/- 12 mumol/g dry wt during 40 minutes of metabolic inhibition. This value was calculated as 41.8% of intracellular potassium in the control heart and agreed with the decrement of intracellular potassium measured by NMR. During the metabolic inhibition and recovery period, a linear correlation was observed between the changes in 39K NMR-observed intracellular potassium and the cumulative potassium loss. The present results evaluate the in...Continue Reading

References

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Citations

Oct 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·B D Foy, D Burstein
Oct 15, 1998·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·N B RadfordA D Sherry
Feb 17, 1993·Molecular and Cellular Biochemistry·T IshibashiS Imai
Dec 13, 2005·European Biophysics Journal : EBJ·Anthony D MaherPhilip W Kuchel

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