Potassium channel dysfunction in hypothalamic glucose-receptive neurones of obese Zucker rats

The Journal of Physiology
I C RoweMichael L J Ashford

Abstract

1. We have shown, using intracellular and cell-attached recordings, that glucose-receptive (GR) neurones of obese Zucker rats exhibit abnormal electrophysiological responses to changes in extracellular glucose concentration, whereas GR neurones of lean Zucker and control rats respond normally. 2. In inside-out recordings from obese rat GR neurones it was shown that the 150 pS ATP-sensitive K+ (KATP) and the 160 pS calcium-activated K+ (KCa) channels were absent, whereas both were present in GR neurones of lean Zucker and control rats. 3. The potassium channel most frequently observed in inside-out patches from obese GR neurones was characterized by a conductance of 213 pS, was activated by raising internal calcium and inhibited by application of internal ATP. This channel (which we have termed Kfa) was not observed in lean or control rat GR neurones. 4. Tolbutamide (100 microM) was found to induce no effect or to elicit a small depolarization of obese rat GR neurones in the absence of glucose, in contrast to its clear excitatory actions on control or lean Zucker GR neurones. 5. Intracellular, cell-attached and inside-out recordings from obese rat non-GR neurones showed that there was no alteration in their membrane properties o...Continue Reading

Citations

Jan 22, 2003·Journal of Neuroendocrinology·T AlquierL Pénicaud
Nov 15, 2005·The Journal of Physiology·Robert H BalfourStefan Trapp
Apr 12, 2016·Frontiers in Pharmacology·Luiz H C VasconcelosBagnólia A Silva
Oct 23, 2013·Molecular and Cellular Endocrinology·Jennifer A ChalmersDenise D Belsham
Sep 28, 2004·Diabetes·Barry E LevinAmbrose A Dunn-Meynell
Aug 13, 2002·Current Opinion in Clinical Nutrition and Metabolic Care·Luc PénicaudElise Guillod
Nov 13, 2014·Hormone Molecular Biology and Clinical Investigation·Lori Asarian, Thomas Bächler
May 8, 1999·The American Journal of Physiology·B E LevinV H Routh
Jan 10, 2018·Frontiers in Neuroscience·Laura L KoekkoekSusanne E la Fleur
Nov 12, 2003·Experimental Biology and Medicine·Katsuhiko NagamoriKazuo Sasaki
Jul 16, 2002·Physiology & Behavior·Vanessa H Routh

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