Potassium channels in human fetal airway smooth muscle cells

Pediatric Research
V A SnetkovJ P Ward

Abstract

Ion channels underlying the resting membrane potential were examined in human fetal airway smooth muscle (ASM). Tissue was obtained from the Medical Research Council Tissue Bank, London, UK. ASM cells were enzymatically dispersed, and ion currents were examined using a patch clamp. Although all cells were of similar size and stained intensely for vimentin, only approximately 50% stained intensely for smooth muscle alpha-actin or myosin heavy chain. Depolarization induced a tetraethylammonium (TEA)- and charybdotoxin (ChTX)-sensitive outward current that varied widely among cells (<50 to >2000 pA at +100 mV), and a smaller nonselective cation current that was similar in all cells (approximately 20 pA at +100 mV). The TEA-sensitive current was associated with three types of large conductance, ChTX-sensitive K+ channel: a 200-pS channel, which was active at negative potentials and low [Ca2+], as described for freshly isolated adult ASM, and two other K+ channels of 100 and 150 pS, previously observed only in adult ASM proliferating in culture. ChTX, but not 4-aminopyridine, caused a substantial depolarization in the current clamp mode, suggesting that, in contrast to ASM from other species or vascular smooth muscle, large conducta...Continue Reading

References

Sep 1, 1975·The Tohoku Journal of Experimental Medicine·K AkasakaC Abe
Feb 1, 1992·The Journal of Physiology·J P BoyleM I Kotlikoff
Jan 1, 1989·The American Review of Respiratory Disease·A L JamesJ C Hogg
Apr 1, 1987·The American Review of Respiratory Disease·N L Stephens
Nov 1, 1982·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·C DavisE E Daniel
Sep 1, 1995·Pflügers Archiv : European journal of physiology·S J KimK W Kim
Aug 1, 1994·The European Respiratory Journal·M P SparrowH W Mitchell
Jul 1, 1995·British Journal of Pharmacology·V A SnetkovJ P Ward
Jul 1, 1995·Physiological Reviews·G K Owens
Apr 1, 1994·Pediatric Clinics of North America·S H Abman, J R Groothius
May 1, 1993·American Journal of Respiratory Cell and Molecular Biology·P B McCray
Dec 1, 1995·The American Journal of Physiology·H K LeeK M Sanders
Sep 1, 1996·Experimental Physiology·V A SnetkovJ P Ward
Jan 1, 1997·The American Journal of Physiology·B K FleischmannM I Kotlikoff

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Citations

May 15, 2001·British Journal of Pharmacology·V A SnetkovJ P Ward
Apr 28, 2006·Pediatric Research·Omar ParvezJaques Belik
Feb 26, 2013·PloS One·Melissa F BreretonSusan L Greenwood
Sep 3, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marco GhionzoliPaolo De Coppi
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Luke J Janssen

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