Potassium chloride inhibits proliferation of cerebellar granule neuron progenitors

Brain Research. Developmental Brain Research
H Cui, R F Bulleit

Abstract

CNS neurogenesis involves a critical transition where neuronal progenitors exit the cell cycle and initiate terminal differentiation. Recent experiments have suggested that depolarization inhibits DNA synthesis in cortical progenitors. Depolarization of proliferating neuronal progenitors may thus activate mechanisms that prevent proliferation and allow the initiation of terminal differentiation. We present evidence that depolarizing concentrations of KCl (25-50 mM) reduce proliferation of developing postnatal cerebellar granule cells in culture. These studies show that KCl antagonizes the mitogenic response of granule cells to insulin-like growth factor-I (IGF-I) and that this reduction in proliferating cells is not the result of a selective cell death. We also examined the differentiation of granule cell cultures using Brn-5 expression as an early differentiation marker. In vivo Brn-5 expression occurs soon after developing granule cells exit the cell cycle and begin their final differentiation. In control cultures and cultures treated with high concentrations of KCl Brn-5 expression increased over 24-48 h of culture. Our results suggest depolarizing concentrations of KCl antagonize proliferation of cerebellar granule neuron p...Continue Reading

References

Oct 11, 1991·Science·S K McConnell, C E Kaznowski
Feb 1, 1985·The Journal of Cell Biology·M E Hatten
Jan 1, 1995·Annual Review of Neuroscience·F H GageL J Fisher
Jan 1, 1997·Journal of Neurobiology·U BechererA Feltz
Apr 18, 1997·Brain Research. Developmental Brain Research·X Lin, R F Bulleit
Jul 30, 1997·Biochemical and Biophysical Research Communications·H Cui, R F Bulleit
Jun 13, 1998·Brain Research. Developmental Brain Research·X LinR F Bulleit
Oct 1, 1961·Experimental Neurology·I L MIALE, R L SIDMAN

❮ Previous
Next ❯

Citations

Feb 12, 2000·Neuroscience·A M StarikovaP G Kostyuk
Mar 14, 2002·Journal of Neuroscience Research·Balázs HerberthEmília Madarász
Mar 17, 2009·Molecular and Cellular Neurosciences·G Le DréauM Laurent
Nov 14, 1998·Biochemical and Biophysical Research Communications·M RoschierA Salminen
Sep 5, 2002·Nature Reviews. Neuroscience·David F Owens, Arnold R Kriegstein
Dec 29, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R Bernabeu, F R Sharp
Oct 12, 2007·Physiological Reviews·Yehezkel Ben-AriRustem Khazipov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.