Potassium deficiency results in accumulation of ultra-high molecular weight poly-beta-hydroxybutyrate in a methane-utilizing mixed culture

Journal of Applied Microbiology
J HelmU Stottmeister

Abstract

To investigate the effect of various single nutrient deficiencies on poly-beta-hydroxybutyrate (PHB) biosynthesis in a methane-utilizing mixed culture (dominant species Methylocystis sp. GB 25 DSM 7674). Poly-beta-hydroxybutyrate accumulation experiments were performed in 7 and 70 l bioreactors and initiated by potassium, sulfur or iron deficiency. After 24 h the PHB content reached levels of 33.6%, 32.6% and 10.4% respectively. Interestingly a polymer with an ultra-high average-weight molecular weight (M(w)) of 3.1 MDa was accumulated under potassium-limited conditions. When sulfur and iron were lacking M(w) were lower by 20.6 and 41.6%. Potassium-deficiency experiments were furthermore characterized by a maximum specific PHB formation rate 0.08 g g(-1)residual biomass (R) h(-1) and a yield coefficient of 0.45 g PHB g(-1) CH(4). Biosynthesis of an ultra-high M(w) PHB in a methane-utilizing mixed culture can be induced by potassium deficiency. This study greatly extends the knowledge in the field of bacterial biopolymer formation with gaseous substrates. The special system used here combines the use of methane a low-cost substrate available from natural and renewable sources with the possibility of employing a mixed-culture in ...Continue Reading

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Citations

Jan 12, 2013·Applied Microbiology and Biotechnology·Kianoush Khosravi-DaraniKenji Tanaka
Jul 11, 2012·Environmental Science & Technology·Katherine H RostkowskiMichael D Lepech
Jul 5, 2011·Applied and Environmental Microbiology·Allison J PiejaCraig S Criddle
Sep 8, 2010·Applied Biochemistry and Biotechnology·Paulo Ivan FernandesGustavo Ribeiro Xavier
Feb 28, 2016·International Journal of Biological Macromolecules·Andrew J CalCharles C Lee
Apr 15, 2014·Biotechnology Advances·Qiang FeiPhilip T Pienkos
Apr 17, 2013·Current Opinion in Biotechnology·Christopher W MarshallHarold D May
Jul 31, 2016·Applied Microbiology and Biotechnology·Hao JiangXin-Hui Xing
Sep 30, 2016·Microorganisms·Peter James StrongSteven Pratt
May 10, 2015·Applied and Environmental Microbiology·Eric R Sundstrom, Craig S Criddle
Oct 30, 2019·The Journal of Microbiology·Rajendra SinghSi Wouk Kim
Feb 28, 2015·Environmental Science & Technology·P J StrongW P Clarke

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