Potassium flux through gramicidin ion channels is augmented in vesicles comprised of plasmenylcholine: correlations between gramicidin conformation and function in chemically distinct host bilayer matrices

Biochemistry
X Chen, R W Gross

Abstract

The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmenylcholine and phosphatidylcholine. The rate constant of gramicidin-mediated K+ ion flux across bilayers comprised of 1-O-(Z)-hexadec-1'-enyl-2-octadec-9'-enoyl-sn-glycero-3-p hos phocholine (plasmenylcholine) was 18.9 +/- 1.7 s-1, while that present across bilayers comprised of 1-hexadecanoyl-2-octadec-9'-enoyl-sn-glycero-3-phosphocholine (phosphatidylcholine) was 12.3 +/- 1.5 s-1. The observed changes were not due to alterations in the nature of the sn-2 aliphatic chain or the net surface charge present at the membrane interface and were unaltered by the addition of several amphiphilic agents (including charged amphiphiles), suggesting that the observed alterations specifically reflect changes in channel function which result from the covalent alteration of host phospholipid in the proximal portion of the sn-1 aliphatic chain (i.e., phospholipid subclass-specific alterations). Addition of cholesterol to bilayer mat...Continue Reading

Citations

Oct 31, 1998·Biochimica Et Biophysica Acta·T C Lee
Dec 17, 2008·Cardiovascular Research·Christopher M JenkinsRichard W Gross
Jul 30, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Megumi NishimukaiHiroshi Hara
Feb 25, 2017·Free Radical Biology & Medicine·A BroniecT Sarna
Mar 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Karl F HeroldHugh C Hemmings
Aug 25, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Agnieszka BroniecHitoshi Matsuki

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