Potassium restriction, high protein intake, and metabolic acidosis increase expression of the glutamine transporter SNAT3 (Slc38a3) in mouse kidney

American Journal of Physiology. Renal Physiology
Stephanie M Busque, Carsten A Wagner

Abstract

Kidneys produce ammonium to buffer and excrete acids through metabolism of glutamine. Expression of the glutamine transporter Slc38a3 (SNAT3) increases in kidney during metabolic acidosis (MA), suggesting a role during ammoniagenesis. Potassium depletion and high dietary protein intake are known to elevate renal ammonium excretion. In this study, we examined SNAT3, phosphate-dependent glutaminase (PDG), and phosphoenolpyruvate carboxykinase (PEPCK) regulation during a control (0.36%) or low-K(+) (0.02%) diet for 7 or 14 days or a control (20%) or high-protein (50%) diet for 7 days. MA was induced in control and low-K(+) groups by addition of NH(4)Cl. Urinary ammonium excretion increased during MA, after 14-day K(+) restriction alone, and during high protein intake. SNAT3, PDG, and PEPCK mRNA abundance were elevated during MA and after 14-day K(+) restriction but not during high protein intake. SNAT3 protein abundance was enhanced during MA (both control and low K(+)), after 14-day low-K(+) treatment alone, and during high protein intake. Seven-day dietary K(+) depletion alone had no effect. Immunohistochemistry showed SNAT3 staining in earlier parts of the proximal tubule during 14-day K(+) restriction with and without NH(4)Cl ...Continue Reading

References

Jun 1, 1977·Kidney International·R L Tannen
Oct 1, 1976·The American Journal of Physiology·R L Tannen, J McGill
Oct 1, 1991·Kidney International·A TizianelloG Deferrari
Mar 1, 1987·The American Journal of Physiology·T D McKinney, K K Davidson
Nov 1, 1986·The American Journal of Physiology·W R AdamM W Weiner
Apr 1, 1986·The American Journal of Physiology·S Sastrasinh, M Sastrasinh
Feb 1, 1969·The Journal of Clinical Investigation·G A Alleyne, G H Scullard
Nov 1, 1970·The Journal of Clinical Investigation·A S Pagliara, A D Goodman
Jun 1, 1973·The American Journal of Physiology·D E Kamm, G L Strope
Apr 1, 1970·The Journal of Clinical Investigation·R L Tannen
Jan 1, 1965·Scandinavian Journal of Clinical and Laboratory Investigation·C Slot
Nov 1, 1983·The American Journal of Physiology·P JaegerG Giebisch
Jun 1, 1994·The American Journal of Clinical Nutrition·T Remer, Friedrich Manz
Nov 14, 1997·The American Journal of Physiology·T C CuriR Curi
Feb 12, 1998·The American Journal of Physiology·D E Wesson
Jun 11, 1999·The American Journal of Physiology·R B Silver, M Soleimani
Aug 15, 2001·American Journal of Physiology. Renal Physiology·N P Curthoys, G Gstraunthaler
Feb 19, 2002·The Journal of Physiology·Angelika BröerStefan Bröer
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Anne M KarinchWiley W Souba
Apr 30, 2003·The American Journal of Clinical Nutrition·T RemerFriedrich Manz
Jul 8, 2003·Pflügers Archiv : European journal of physiology·Bryan Mackenzie, Jeffrey D Erickson
Sep 1, 1954·The American Journal of Physiology·M IACOBELLISG E GRIFFIN
Nov 26, 2003·Journal of the American Society of Nephrology : JASN·Paul A StehbergerCarsten A Wagner
Dec 6, 2003·Acta Physiologica Scandinavica·Susan M Wall
Sep 1, 2004·Cell Biochemistry and Function·Tania Cristina Pithon-CuriRui Curi
Sep 2, 2004·Journal of the American Society of Nephrology : JASN·Apurv KhannaDonald E Wesson
Sep 7, 2006·American Journal of Physiology. Renal Physiology·Anne M KarinchWiley W Souba
Sep 28, 2006·American Journal of Physiology. Renal Physiology·Caroline MoretCarsten A Wagner
Dec 7, 2007·Physiological Genomics·Marta NowikCarsten A Wagner
Apr 18, 2008·Journal of Molecular Neuroscience : MN·Björn E SundbergRobert Fredriksson

Citations

Apr 3, 2012·Histochemistry and Cell Biology·Natascha LierJohannes Loffing
Nov 7, 2013·Pflügers Archiv : European journal of physiology·Stefan Bröer
Dec 1, 2012·American Journal of Physiology. Renal Physiology·Hyun-Wook LeeI David Weiner
Dec 12, 2012·American Journal of Physiology. Renal Physiology·Jesse M BishopI David Weiner
Jul 6, 2013·Nutrition Research·Caroline A GunnMarlena C Kruger
Feb 27, 2013·Advances in Chronic Kidney Disease·Julia J Scialla, Cheryl A M Anderson
Oct 12, 2010·Kidney International·Carsten A WagnerPascal Houillier
Dec 20, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Remy BürkiCarsten A Wagner
Apr 22, 2015·The Journal of Clinical Investigation·P Richard GrimmPaul A Welling
Dec 17, 2011·Respiratory Physiology & Neurobiology·Nina K IversenTobias Wang
May 14, 2016·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·I David Weiner
Jul 1, 2016·Channels·Isabel Rubio-Aliaga, Carsten A Wagner
Mar 3, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nadine RuderischFrancois Verrey
Sep 9, 2017·Rejuvenation Research·Patrizia D'AquilaGiuseppe Passarino
Feb 3, 2017·Physiological Reviews·I David Weiner, Jill W Verlander
Mar 22, 2014·Journal of the American Society of Nephrology : JASN·Lisa BounoureCarsten A Wagner
Aug 1, 2014·Clinical Journal of the American Society of Nephrology : CJASN·I David WeinerJeff M Sands
Jul 29, 2011·American Journal of Physiology. Renal Physiology·Shaikh Abu HossainHassane Amlal
Mar 22, 2014·American Journal of Physiology. Renal Physiology·I David Weiner, Jill W Verlander
Dec 17, 2019·American Journal of Physiology. Renal Physiology·Hyun-Wook LeeI David Weiner
Mar 25, 2016·American Journal of Physiology. Renal Physiology·Hyun-Wook LeeI David Weiner
Feb 6, 2019·Scientific Reports·Alexandra LahtinenTiina Paunio
Aug 8, 2019·American Journal of Physiology. Renal Physiology·Autumn N HarrisI David Weiner
Jul 14, 2019·Seminars in Nephrology·Carsten A WagnerSoline Bourgeois

Related Concepts

Slc38a3 protein, mouse
Metabolic Acidosis
Ammonium Chloride
Quaternary Ammonium Compounds
Casein A
Phosphate-Activated Glutaminase
Kidney
Kidney Tubules, Proximal
GTP-Dependent Phosphoenolpyruvate Carboxykinase
Potassium Deficiency

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