PMID: 36910May 3, 1979

Potassium-stimulated ATPase activity and hydrogen transport in gastric microsomal vesicles

Biochimica Et Biophysica Acta
H C LeeJ G Forte

Abstract

The Mg2+-dependent, K+-stimulated ATPase of microsomes from pig gastric mucosa has been studied in relation to observed active H+ transport into vesicular space. Uptake of fluorescent dyes (acridine orange and 9-aminoacridine) was used to monitor the generated pH gradient. Freeze-fracture electron microscopy showed that the vesicular gastric microsomes have an asymmetric distribution of intramembraneous particles (P-face was particulate; E-face was relatively smooth. Valinomycin stimulated both dye uptake and K+-ATPase (valinomycin-stimulated K+-ATPase); stimulation by valinomycin was due to increased K+ entry to some intravesicular activating site, which in turn depends upon the accompanying anion. Using the valinomycin-stimulated K+-ATPase and H+ accumulation as an index, the sequence for anion permeation was NO-3 greater than Br- greater than Cl- greater than I- greater than acetate approximately isethionate. When permeability to both K+ and H+ was increased (e.g using valinomycin plus a protonophore or nigericin), stimulation of K+-ATPase was much less dependent on the anion and the observed dissipation of the vesicular pH gradient was consistent with an 'uncoupling' of ATP hydrolysis from H+ accumulation. Thiocyanate inter...Continue Reading

References

Jan 1, 1983·The Journal of Membrane Biology·J M Diamond, T E Machen
Jan 1, 1981·The Journal of Membrane Biology·W W Reenstra, J G Forte
Jan 1, 1983·The Journal of Membrane Biology·J M Wolosin, J G Forte
Apr 15, 1981·The Journal of Membrane Biology·D J Culp, J G Forte
Jun 1, 1988·Biochemical Pharmacology·D J KeelingJ Senn-Bilfinger
Jun 21, 1991·Biochemical Pharmacology·D J KeelingC A Leach
May 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A A López MañanesF V Vega
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R W Van DykeB F Scharschmidt
Sep 1, 1991·Cell Regulation·H A Thomas, T E Machen
Jul 2, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Wenjun HeLixin Zhu
Jun 1, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Anand RotteFlorian Lang
Jun 17, 2010·Journal of Gastroenterology·Anand RotteFlorian Lang
Nov 30, 2010·Journal of Gastroenterology·Venkanna PashamFlorian Lang
Jan 1, 1989·Annals of the New York Academy of Sciences·J G ForteJ M Wolosin
May 19, 1982·Biochimica Et Biophysica Acta·R J Kessler, D D Fanestil
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Anand RotteFlorian Lang
Jan 1, 1980·Annals of the New York Academy of Sciences·H C LeeJ G Forte
Jan 1, 1980·Annals of the New York Academy of Sciences·S L BontingJ J Schrijen
Jan 1, 1980·Annals of the New York Academy of Sciences·G SachsG Saccomani
Jan 1, 1982·Annals of the New York Academy of Sciences·R B DicksonI H Pastan
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·Juan C Del ValleJuan E Felíu
Dec 25, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·María SalvatellaJuan E Felíu
Oct 12, 2007·Physiology·Dirk Heitzmann, Richard Warth
May 1, 1984·The American Journal of Physiology·J M Wolosin, J G Forte

Citations

Jul 1, 1969·The Journal of Cell Biology·D W Deamer, R J Baskin
Jul 30, 1974·Biochemistry·G Inesi, D Scales
Apr 25, 1973·Biochimica Et Biophysica Acta·A L Ganser, J G Forte
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D Branton
Mar 2, 1963·Nature·J G ForteR E DAVIES

Related Concepts

Thermodynamics
Biochemical Pathway
Bromides
Tissue Membrane
Aminacrine
Adenosine Triphosphatases
Microsomes
Valinomycin
Potassium
Structure of Pyloric Gland

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