Potato Cyst Nematodes: A New Threat to Potato Production in East Africa

Frontiers in Plant Science
Harrison MburuDanny Coyne

Abstract

Potato cyst nematodes (PCN), such as Globodera rostochiensis and Globodera pallida, are quarantine restricted pests of potato causing major yield and financial losses to farmers. G. rostochiensis was first reported from Kenya's key potato growing area in 2015. We sought to determine the diversity, prevalence and distribution of PCN species across the country by conducting a country-wide survey between 2016 and 2018, which included a more focused, follow-up assessment in three key potato growing counties. A total of 1,348 soil samples were collected from 20 potato growing counties. Information regarding local potato farming practices, potato cultivar use, their diversity and availability was also recorded. PCN cysts were obtained from 968 samples (71.8%) in all the counties surveyed, with Nyandarua County recording the highest PCN field-incidence at 47.6%. The majority of PCN populations, 99.9%, were identified as G. rostochiensis, while G. pallida was recovered from just one field, in a mixed population with G. rostochiensis. Inconsistencies in PCR amplification efficiency was observed for G. rostochiensis using the recommended EPPO primers, compared with ITS primers AB28/TW81, indicating that this protocol cannot be entirely r...Continue Reading

References

May 20, 2015·Methods in Molecular Biology·Alex ReidJon Pickup
Jun 7, 2019·Plant Disease·Benjamin MimeeGuy Bélair

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Datasets Mentioned

BETA
MN378644.1
MN378550.1
MG309873.1
MG309920.1
MN382342.1
MN378566.1

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
Excel
BioEdit Sequence Alignment Editor
lsmeans
psych
R
Seaview
Epicollect

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