PMID: 9161042Apr 1, 1997Paper

Potato guard cells respond to drying soil by a complex change in the expression of genes related to carbon metabolism and turgor regulation

The Plant Journal : for Cell and Molecular Biology
J KopkaB Müller-Röber

Abstract

Altering stomatal function by a guard cell-targeted transgenic approach with the aim of increased stress tolerance and crop yield requires knowledge of the natural fluctuations of stomatal gene expression under stress conditions. We developed a fast method for the isolation of RNA from epidermal fragments of potato leaves (Solanum tuberosum L. cv. Désirée), demonstrated that this RNA preparation is highly enriched in guard cell transcripts and used this method to investigate the response of gene expression in guard cells to mild drought stress. Drought was applied in planta by withholding water over a period of 2-4 days. In the following work responses observed under these conditions are called 'long-term' in contrast to immediate (short-term) stomatal opening and closing responses to environmental stress. We observed both gene-specific increases and decreases of steady-state transcript levels. In particular, the mRNA levels of sucrose synthase and sucrose-phosphate synthase were elevated 5.5-fold and 1.4-fold, respectively. In contrast, expression of an inwardly rectifying K+ channel from guard cells (kst1) and of a plasma membrane H(+)-ATPase (pha2) was reduced to 26% and 36%, respectively, of the expression in watered contro...Continue Reading

Citations

Jun 24, 2006·The Journal of Membrane Biology·F Gambale, N Uozumi
Jul 6, 2001·The Plant Journal : for Cell and Molecular Biology·A A WebbA M Hetherington
Dec 12, 2001·The Plant Journal : for Cell and Molecular Biology·G PleschB Mueller-Roeber
Feb 19, 2002·The Plant Journal : for Cell and Molecular Biology·S ZimmermannB Mueller-Roeber
Nov 27, 2008·Journal of Experimental Botany·Michael J GardnerAlex A R Webb
Aug 25, 2009·Journal of Experimental Botany·Tou Cheu XiongCarl K-Y Ng
Dec 11, 2007·Plant Physiology·Irene BaroliSusanne von Caemmerer
Sep 25, 2003·Annual Review of Plant Biology·Anne-Aliénor Véry, Hervé Sentenac
Jan 11, 2007·Annual Review of Plant Biology·Ken-ichiro ShimazakiToshinori Kinoshita
Nov 18, 2011·BMC Plant Biology·Eleonora CominelliChiara Tonelli
May 23, 2012·Plant Molecular Biology·Carolina Gimiliani LembkeGlaucia Mendes Souza
Nov 21, 2012·New Biotechnology·Eleonora CominelliMassimo Galbiati
Feb 22, 2005·The New Phytologist·Alain Vavasseur, Agepati S Raghavendra
Aug 18, 2009·The Plant Journal : for Cell and Molecular Biology·Olivia WilkinsMalcolm M Campbell
Mar 29, 2003·The Plant Journal : for Cell and Molecular Biology·Lee HuntJulie E Gray
Apr 16, 2008·The Plant Journal : for Cell and Molecular Biology·Jin Sun KimHunseung Kang
Dec 17, 2008·The New Phytologist·Tracy Lawson
Apr 1, 2008·The New Phytologist·Pengtao Wang, Chun-Peng Song
Oct 18, 2011·Plant, Cell & Environment·Werner C AntunesMarcelo E Loureiro
Apr 30, 2014·The Plant Journal : for Cell and Molecular Biology·Zhong Jie DingShao Jian Zheng
Feb 4, 2003·Anais Da Academia Brasileira De Ciências·Marcelo J SurpiliLothar Willmitzer
Aug 16, 2000·Journal of Experimental Botany·K AghoramC W Levenson
Apr 20, 2006·Plant & Cell Physiology·Michio DoiKen-Ichiro Shimazaki
Dec 1, 2020·Plant, Cell & Environment·Bastian Leander FranziskyChristian Zörb
Mar 1, 2002·The New Phytologist·Alex A R Webb, Andrew J Baker
Jan 7, 2004·Journal of Agricultural and Food Chemistry·Yung-Liang WangHsien-Yi Sung

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