PMID: 9420234Jan 7, 1998Paper

Potato leafroll virus binds to the equatorial domain of the aphid endosymbiotic GroEL homolog

Journal of Virology
S A HogenhoutJ F van den Heuvel

Abstract

A GroEL homolog with a molecular mass of 60 kDa, produced by the primary endosymbiotic bacterium (a Buchnera sp.) of Myzus persicae and released into the hemolymph, has previously been shown to be a key protein in the transmission of potato leafroll virus (PLRV). Like other luteoviruses and pea enation mosaic virus, PLRV readily binds to extracellular Buchnera GroEL, and in vivo interference in this interaction coincides with reduced capsid integrity and loss of infectivity. To gain more knowledge of the nature of the association between PLRV and Buchnera GroEL, the groE operon of the primary endosymbiont of M. persicae (MpB groE) and its flanking sequences were characterized and the PLRV-binding domain of Buchnera GroEL was identified by deletion mutant analysis. MpB GroEL has extensive sequence similarity (92%) with Escherichia coli GroEL and other members of the chaperonin-60 family. The genomic organization of the Buchnera groE operon is similar to that of the groE operon of E. coli except that a constitutive promoter sequence could not be identified; only the heat shock promoter was present. By a virus overlay assay of protein blots, it was shown that purified PLRV bound as efficiently to recombinant MpB GroEL (expressed i...Continue Reading

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Citations

Apr 13, 2000·Insect Molecular Biology·K Stanley, B Fenton
Mar 1, 2003·Molecular Plant Pathology·Michael TalianskyHugh Barker
Aug 6, 2008·Annual Review of Phytopathology·Saskia A HogenhoutMargaret G Redinbaugh
Apr 5, 2013·Biological Reviews of the Cambridge Philosophical Society·Brian HendersonPeter A Lund
Sep 5, 2012·Molecular Genetics and Genomics : MGG·Punya NachappaCecilia Tamborindeguy
Nov 18, 2020·Plants·Muhammad Salman MubarikMohamed Soliman Elshikh
Jun 14, 2000·Current Opinion in Microbiology·N A Moran, P Baumann

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