PMID: 9177310May 1, 1997Paper

Potato SNF1-related protein kinase: molecular cloning, expression analysis and peptide kinase activity measurements

Plant Molecular Biology
A L ManN G Halford

Abstract

A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-related genes in the potato genome. PKIN503 was used to screen a tuber cDNA library and a genomic library, and one cDNA and five genomic clones were isolated. The nucleotide sequences of a portion of all five genomic clones were shown to be identical and only one, pgPKIN1, was analysed further. The cDNA was found to be truncated at the 5' end but the cDNA and genomic sequences contained only 15 substitutions, two of which resulted in changes in the derived amino acid sequence. PKIN1 was shown to encode an Mr 57,854 protein with 61-70% sequence similarity with other plant SNF1-related protein kinases. Northern blot analysis revealed some tissue-specific differences in PKIN1 transcript levels, the lowest being detected in leaves and the highest in stolons. However, much greater differences were found in SNF1-related activity, which was measured using a phosphorylation assay with a substrate peptide which has been shown pre...Continue Reading

Citations

Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Agnes LovasZsófia Bánfalvi
Jul 13, 2004·Plant Biology·L BorisjukH Weber
May 20, 2009·FEBS Letters·Cristina López-PazVictoria Lumbreras
Nov 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Michael A HardiganC Robin Buell
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·J P BoulyM Thomas
Jan 1, 2003·Journal of Experimental Botany·Nigel G HalfordYuhua Zhang
Apr 2, 2004·The EMBO Journal·Mattias ThelanderHans Ronne
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·L LakatosZ Bánfalvi
Mar 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rizwan Elahi, Tai-Hua Mu
Dec 1, 2006·Plant Biotechnology Journal·Nigel G Halford, Matthew J Paul
May 20, 2018·Biochimica Et Biophysica Acta. Proteins and Proteomics·Dongyin Su, Timothy P Devarenne
May 14, 1999·Trends in Plant Science·N G HalfordD G Hardie

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