Potent activation of mitochondria-mediated apoptosis and arrest in S and M phases of cancer cells by a broccoli sprout extract.

Molecular Cancer Therapeutics
Li TangJed W Fahey

Abstract

We have previously shown that broccoli sprouts are a rich source of chemopreventive isothiocyanates, which potently induce carcinogen-detoxifying enzymes and inhibit the development of mammary and skin tumors in rodents. However, the principal isothiocyanate present in broccoli sprout extracts, sulforaphane, not only induces carcinogen-detoxifying enzymes but also activates apoptosis and blocks cell cycle progression. In this article, we show that an aqueous extract of broccoli sprouts potently inhibits the growth of human bladder carcinoma cells in culture and that this inhibition is almost exclusively due to the isothiocyanates. Isothiocyanates are present in broccoli sprouts as their glucosinolate precursors and blocking their conversion to isothiocyanates abolishes the antiproliferative activity of the extract. Moreover, the potency of isothiocyanates in the extract in inhibiting cancer cell growth was almost identical to that of synthetic sulforaphane, as judged by their IC50 values (6.6 versus 6.8 micromol/L), suggesting that other isothiocyanates in the extract may be biologically similar to sulforaphane and that nonisothiocyanate substances in the extract may not interfere with the antiproliferative activity of the isot...Continue Reading

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Citations

Jan 26, 2010·Nutrition and Cancer·Chih-Ping HsuShao-Cheng Wang
Aug 29, 2007·Acta Pharmacologica Sinica·Yuesheng Zhang, Li Tang
Jun 17, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Li TangSusan E McCann
May 9, 2014·Cell Biochemistry and Biophysics·Pradip K KamatNeetu Tyagi
Dec 20, 2011·Mutation Research·Carmela FimognariPatrizia Hrelia
Jun 8, 2007·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Jay H Fowke
May 6, 2008·Free Radical Biology & Medicine·Numsen HailJulian E Spallholz
Dec 8, 2011·Molecular Nutrition & Food Research·Lynnette R Ferguson, Ralf C Schlothauer
Aug 15, 2015·Current Pharmacology Reports·Omkara L VeerankiYuesheng Zhang
Dec 10, 2016·Molecular Nutrition & Food Research·Jed W FaheyKatherine K Stephenson
Aug 7, 2018·Molecular Nutrition & Food Research·Besma AbbaouiAmir Mortazavi
Sep 30, 2017·Pharmacological Reports : PR·Monika PrełowskaAnna Herman-Antosiewicz
Mar 4, 2008·Cancer Research·Rex MundayYuesheng Zhang
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muna Ali AbdallaKarl H Mühling
Jan 19, 2021·Journal of Oral and Maxillofacial Pathology : JOMFP·D B NandiniPraveen B Reddy
Aug 8, 2021·International Journal of Molecular Sciences·Paulina WignerJoanna Saluk-Bijak

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