Potent anti-HIV activity of scytovirin domain 1 peptide

Peptides
Changyun XiongJames B McMahon

Abstract

Scytovirin (SVN) is a novel anti-HIV protein isolated from aqueous extracts of the cultured cyanobacterium Scytonema varium. SVN contains two apparent domains, one comprising amino acids 1-48 and the second stretching from amino acids 49 to 95. These two domains display significant homology to each other and a similar pattern of disulfide bonds. Two DNA constructs encoding scytovirin 1-48 (Cys7Ser) (SD1) and 49-95 (Cys55Ser) (SD2) were constructed, and expressed in E. coli, with thioredoxin fused to their N-terminus. Purified recombinant products were tested for binding activities with the HIV surface envelope glycoproteins gp120 and gp41. Whole cell anti-HIV data showed that SD1 had similar anti-HIV activity to the full-length SVN, whereas SD2 had significantly less anti-HIV activity. Further deletion mutants of the SD1 domain (SVN(3-45)Cys7Ser, SVN(6-45)Cys7Ser, SVN(11-45)Cys7Ser) showed that the N-terminal residues are necessary for full anti-HIV activity of SD1 and that an eight amino acid deletion from the C-terminus (SVN(1-40)Cys7Ser) had a significant effect, decreasing the anti-HIV activity of SD1 by approximately five-fold.

References

Jun 1, 1991·Journal of Virological Methods·R J GulakowskiM R Boyd
Oct 27, 2001·Journal of Natural Products·B R O'Keefe
Nov 30, 2002·Expert Opinion on Investigational Drugs·Ray Y ChenMichael S Saag
Mar 5, 2003·Biochemistry·Heidi R BokeschMichael R Boyd
Mar 15, 2003·The New England Journal of Medicine·Jacob P LalezariUNKNOWN TORO 1 Study Group
Jan 27, 2004·Antimicrobial Agents and Chemotherapy·Jeffrey M JacobsonWilliam C Olson
Dec 2, 2004·Progress in Biophysics and Molecular Biology·Istvan Botos, Alexander Wlodawer
Jan 11, 2005·Protein and Peptide Letters·Alexander M Cole
Jul 16, 2005·Current Eye Research·Y Jerold GordonAlison M McDermott
Sep 27, 2005·Drug Discovery Today·Hugues J-P Ryser, Rudolf Flückiger

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Citations

Mar 28, 2013·Antonie van Leeuwenhoek·Rakhi Bajpai Dixit, M R Suseela
Apr 7, 2011·The Journal of Antibiotics·Rahul Kunwar SinghTribhuban M Mohapatra
Apr 17, 2007·Journal of Molecular Biology·Robert L McFeetersR Andrew Byrd
Oct 30, 2007·Protein Science : a Publication of the Protein Society·Tinoush MoulaeiAlexander Wlodawer
Jun 24, 2010·Protein Science : a Publication of the Protein Society·Tinoush MoulaeiAlexander Wlodawer
Jun 26, 2010·Medicinal Research Reviews·K O François, J Balzarini
Mar 18, 2011·Reviews in Medical Virology·Kevin K AriënGuido Vanham
Mar 1, 2015·Expert Opinion on Therapeutic Targets·Priyamvada AcharyaPeter D Kwong
Apr 25, 2017·International Journal of Biological Macromolecules·Ram Sarup SinghJohn F Kennedy
Aug 2, 2017·Molecular Biology Reports·Andrei Santos SiqueiraEvonnildo Costa Gonçalves
Nov 10, 2020·Journal of Biomolecular Structure & Dynamics·Mabroka H SaadNagwa M Sidkey
Mar 23, 2021·Journal of Biomolecular Structure & Dynamics·Samaneh Jafari PorzaniBahareh Nowruzi

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