Potent Clastogenicity of Bisphenol Compounds in Mammalian Cells-Human CYP1A1 Being a Major Activating Enzyme.

Environmental Science & Technology
Hang YuYungang Liu

Abstract

Bisphenols (BPs) are environmental pollutants with relevant DNA damage in human population; however, they are generally inactive in standard mutagenicity assays, possibly due to insufficient metabolic activation. In this study, induction of micronuclei and double-strand DNA breaks by BPA, BPF, and BPS in Chinese hamster V79-derived cell lines expressing various human CYP enzymes and a human hepatoma (C3A) (metabolism-proficient) cell line were investigated. Molecular docking of BPs to human CYPs indicated some substrate-enzyme potentials, including CYP1A1 for each compound, which did not induce micronuclei in V79-derived cell lines expressing human CYP1A2, 2E1, or 3A4 but became positive in human CYP1A1-expressing (V79-hCYP1A1) cells. In V79-hCYP1A1 and C3A cells, all compounds induced double-strand DNA breaks and micronuclei formation, which were blocked/significantly attenuated by 1-aminobenzotriazole (CYP inhibitor) or 7-hydroxyflavone (selective CYP1A1 inhibitor). Coexposure of C3A cells to pentachlorophenol (sulfotransferase 1 inhibitor) or ketoconazole (UDP-glucuronosyltransferase 1A inhibitor) potentiated micronuclei induction by each compound, with thresholds lowered from 2.5-5.0 to 0.6-1.2 μM. Immunofluorescence staini...Continue Reading

References

Jul 1, 1995·European Journal of Pharmacology·W A SchmalixH Greim
Sep 18, 2001·Biological & Pharmaceutical Bulletin·T NiwaM Katagiri
Sep 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wei Peng YongMark J Ratain
Dec 20, 2005·Chemical Research in Toxicology·Robert T KinobeElizabeth M J Gillam
Sep 11, 2007·Reproductive Toxicology·Laura N VandenbergWade V Welshons
Jan 17, 2008·Environmental Health Perspectives·Antonia M CalafatLarry L Needham
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Jun 6, 2009·The International Journal of Biochemistry & Cell Biology·Michelle W M LiC Yan Cheng
Aug 4, 2010·Environmental Pollution·Pingqing Fu, Kimitaka Kawamura
Sep 30, 2010·Environmental Health Perspectives·John D MeekerRuss Hauser
Oct 14, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Yuping LiuRenke Dai
Nov 30, 2010·Archives of Toxicology·Onur Kenan Ulutaşİsmet Çok
Feb 22, 2011·Biotechnology and Bioengineering·Jean Matthieu ProtEric Leclerc
Mar 9, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jan SchmidtLucija Peterlin Mašič
Jul 4, 2013·Arhiv za higijenu rada i toksikologiju·Anja FicLucija Peterlin Mašič
Jul 27, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·XinNan HuGongHua Tao
May 5, 2016·Environmental Science & Technology·Da ChenMargaret Widelka
Sep 18, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Hansi JiaYungang Liu
Oct 13, 2016·Environment International·Yanshan LvRuifang Fan
Oct 31, 2016·Environmental Toxicology and Pharmacology·Darja Gramec Skledar, Lucija Peterlin Mašič
Dec 14, 2016·International Journal of Molecular Medicine·Xiaoqian ZhangLanjuan Li
Dec 16, 2017·Environmental Science & Technology·Hangbiao JinZongwei Cai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.