Potent inhibition by trivalent cations of ATP-gated channels

European Journal of Pharmacology
K NakazawaY Ohno

Abstract

The effects of La3+ and other trivalent cations on ATP-gated channels (P2X purinoceptor/channels) were investigated using rat pheochromocytoma PC12 cells and Xenopus oocytes expressing these channels. La3+, Gd3+, Ce3+ and Nd3+ (30-300 microM) inhibited an inward current activated by 30 microM ATP in PC12 cells. The concentration-response curve for the ATP-activated current was shifted by La3+ or Gd3+ toward a higher concentration range, and the slope of the curve became steeper, suggesting the inhibition is non-competitive. La3+ or Gd3+ did not affect the current component that was slowly activated upon hyperpolarization, and selectively inhibited the remaining 'voltage-independent' component. La3+ and Gd3+ also inhibited currents mediated through P2X1 and P2X2 purinoceptors expressed in Xenopus oocytes. The results suggest that La3+ and other trivalent cations inhibit P2X purinoceptors at low concentrations. The inhibition may at least partly be attributed to an allosteric inhibition.

References

May 14, 1992·European Journal of Pharmacology·K InoueA Takanaka
Apr 1, 1991·Pflügers Archiv : European journal of physiology·K NakazawaA Takanaka
Aug 1, 1989·The American Journal of Physiology·E HonoréJ Mironneau
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jul 1, 1994·Pflügers Archiv : European journal of physiology·K NakazawaK Inoue
Feb 1, 1993·Pflügers Archiv : European journal of physiology·K NakazawaK Inoue
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C LiF F Weight
Jul 1, 1993·Pflügers Archiv : European journal of physiology·R ClouesD A Brown
Jan 1, 1994·Pharmacology & Therapeutics·M P Abbracchio, G Burnstock
Nov 15, 1994·European Journal of Pharmacology·K NakazawaK Inoue
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·N InagakiS Seino
Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim
Mar 1, 1993·The Journal of General Physiology·K Nakazawa, P Hess
Jan 18, 1996·European Journal of Pharmacology·K Nakazawa, Y Ohno
Mar 1, 1996·British Journal of Pharmacology·M IkedaK Inoue

❮ Previous
Next ❯

Citations

Aug 9, 2002·European Journal of Pharmacology·Ken NakazawaYasuo Ohno
Jun 8, 2001·Archives of Medical Research·J V Tapia-Vieyra, J Mas-Oliva
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Luis A CeaJuan C Sáez
Jul 9, 2011·Pharmacological Reviews·Claudio CoddouStanko S Stojilkovic
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·R A North, A Surprenant
Oct 28, 2010·British Journal of Pharmacology·Richard J Evans
Jul 4, 2003·British Journal of Pharmacology·Vladimir S VorobjevHelmut L Haas
May 20, 2008·Cell Calcium·Melanie J LudlowSteven J Ennion
Feb 26, 2011·Journal of Pharmacological Sciences·Megumi SugiharaMasato Hirata
Jan 19, 2000·The Journal of Physiology·S Ding, F Sachs
Mar 11, 2009·Neuroscience·M A CrumlingR K Duncan
Sep 12, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Scot L WintersRichard C Boucher
Mar 6, 2010·Platelets·Hu Hu, Marc F Hoylaerts
Apr 8, 2004·American Journal of Physiology. Renal Physiology·Shen-Ling XiaCharles S Wingo
Sep 25, 2002·Physiological Reviews·R Alan North
Jun 12, 2020·International Journal of Molecular Sciences·Elisa BalboaJuan C Sáez
Aug 4, 1998·The American Journal of Physiology·R A CaldwellC M Baumgarten
Nov 29, 2002·Journal of Cell Science·Douglas B LightNicole L Baumann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.