Potent neutralization of SARS-CoV-2 in vitro and in an animal model by a human monoclonal antibody.

BioRxiv : the Preprint Server for Biology
Wei LiDimiter S Dimitrov

Abstract

Effective therapies are urgently needed for the SARS-CoV-2/COVID19 pandemic. We identified panels of fully human monoclonal antibodies (mAbs) from eight large phage-displayed Fab, scFv and VH libraries by panning against the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) glycoprotein. One high affinity mAb, IgG1 ab1, specifically neutralized live SARS-CoV-2 with exceptional potency as measured by two different assays. It competed with human angiotensin-converting enzyme 2 (hACE2) for binding to RBD suggesting a competitive mechanism of virus neutralization. IgG1 ab1 protected transgenic mice expressing hACE2 from high-titer intranasal SARS-CoV-2 challenge (10 5 plaque forming units). Another antibody, VH ab5 did not compete with hACE2 and ab1, and did not neutralize SARS-CoV-2 although its affinity was comparable to that of ab1. The ab1 sequence has relatively low number of somatic mutations indicating that ab1-like antibodies could be quickly elicited during natural SARS-CoV-2 infection or by RBD-based vaccines. IgG1 ab1 does not have developability liabilities, and thus has potential for therapy and prophylaxis of SARS-CoV-2 infections. The rapid identification (within 6 days) of potent mAbs shows the value of larg...Continue Reading

Citations

Sep 18, 2020·Expert Opinion on Therapeutic Targets·Shibo JiangLanying Du
Sep 24, 2020·Signal Transduction and Targeted Therapy·Fei YuShibo Jiang
Jul 15, 2020·Science·Meng YuanIan A Wilson
Oct 8, 2020·Critical Care : the Official Journal of the Critical Care Forum·Salleh N EhaidebAbderrezak Bouchama
Oct 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anum GlasgowJames A Wells
May 1, 2021·Pharmaceuticals·Beata Zalewska-Piątek, Rafał Piątek
May 11, 2021·Frontiers in Molecular Biosciences·Long Min, Qiu Sun
Jun 18, 2021·Frontiers in Molecular Biosciences·Arnaud John Kombe KombeTengchuan Jin

❮ Previous
Next ❯

Methods Mentioned

BETA
phage display
ELISA
biolayer interferometry
transfection
Assay
dynamic
size exclusion chromatography

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Wei LiDimiter S Dimitrov
Proceedings of the National Academy of Sciences of the United States of America
Jian ShangFang Li
Meditsinskaia tekhnika
B M Kotenev
© 2021 Meta ULC. All rights reserved