Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike

Nature
Lihong LiuDavid D Ho

Abstract

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic continues, with devasting consequences for human lives and the global economy1,2. The discovery and development of virus-neutralizing monoclonal antibodies could be one approach to treat or prevent infection by this coronavirus. Here we report the isolation of sixty-one SARS-CoV-2-neutralizing monoclonal antibodies from five patients infected with SARS-CoV-2 and admitted to hospital with severe coronavirus disease 2019 (COVID-19). Among these are nineteen antibodies that potently neutralized authentic SARS-CoV-2 in vitro, nine of which exhibited very high potency, with 50% virus-inhibitory concentrations of 0.7 to 9 ng ml-1. Epitope mapping showed that this collection of nineteen antibodies was about equally divided between those directed against the receptor-binding domain (RBD) and those directed against the N-terminal domain (NTD), indicating that both of these regions at the top of the viral spike are immunogenic. In addition, two other powerful neutralizing antibodies recognized quaternary epitopes that overlap with the domains at the top of the spike. Cryo-electron microscopy reconstructions of one antibody that targets the RBD, a second that target...Continue Reading

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Datasets Mentioned

BETA
MT230904.1

Methods Mentioned

BETA
flow cytometry
enzyme-linked immunosorbent assays
transfection
ELISA
PCR
chip
Assay
FACS

Key Resources (RRID) Mentioned

SCR_016740

Software Mentioned

Molprobity
GraphPad Prism
Leginon
Topaz
COOT
CLUSTALO
ISOLDE
UCSF Chimera
EMRinger
FlowJo

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