Potent protective cellular immune responses generated by a DNA vaccine encoding HSV-2 ICP27 and the E. coli heat labile enterotoxin.

Vaccine
Joel R HaynesL G Payne

Abstract

A mouse model was employed to evaluate protective cellular immune responses induced by an immediate early antigen of HSV-2. Particle-mediated DNA vaccination of mice with a DNA plasmid-encoding ICP27 resulted in the induction of ICP27-specific IFN-gamma and TNF-alpha production in Balb/c mice, but little protection to intranasal challenge with wild type HSV-2. However, when the DNA vaccine was supplemented with as little as 50ng of a vector encoding the A and B subunits of the Escherichia coli heat labile enterotoxin (LT), animals were profoundly protected from morbidity and mortality. The ICP27+LT-mediated protection was correlated with a large increase in ICP27-specific IFN-gamma and TNF-alpha production but cytokine-specific monoclonal antibody treatment at the time of challenge showed that protection was mediated predominantly by IFN-gamma. Furthermore, depletion of T cell subsets prior to infectious challenge demonstrated that removal of either CD8+ or CD4+ T cells impaired protection with CD8+ T cells appearing to play a direct effector role. These data demonstrate that augmented cellular immune responses resulting from LT vector plus antigen vector administration to the skin are biologically significant, leading to enhan...Continue Reading

References

Apr 1, 1996·Immunological Reviews·T M KündigR M Zinkernagel
Oct 31, 1996·Annals of the New York Academy of Sciences·M MarinaroJ R McGhee
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C M PosavadL Corey
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C S MillerR J Jacob
Jun 3, 2000·Annual Review of Immunology·D TortorellaH L Ploegh
Aug 30, 2000·Microbes and Infection·S P DeshpandeB T Rouse
Sep 5, 2002·Reviews in Medical Virology·Susanne BeckebaumGuido Gerken
Jan 15, 2003·Clinical Microbiology Reviews·David M Koelle, Lawrence Corey
Feb 11, 2003·Cellular Immunology·Kaustuv BanerjeeBarry T Rouse
Jul 17, 2003·Journal of Neuroimmunology·Crystal S AnglenRobert H Bonneau
Jul 30, 2003·European Journal of Immunology·Christina BartholdyAllan Randrup Thomsen
Jun 4, 2004·Expert Opinion on Biological Therapy·Joel R Haynes
Aug 18, 2004·The Journal of Clinical Investigation·Jay A BerzofskyIgor M Belyakov
Sep 10, 2005·Vaccine·Robert J DrapeHansi J Dean
Feb 21, 2014·Journal of Virology·Christine JohnstonLawrence Corey

❮ Previous
Next ❯

Citations

Sep 3, 2009·Expert Review of Vaccines·Eric J YagerDeborah Heydenburg Fuller
Feb 28, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Manuel E EngelhornJedd D Wolchok
Apr 22, 2011·Clinical and Vaccine Immunology : CVI·Kenneth C BagleyTimothy R Fouts

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.