PMID: 6160590Oct 1, 1980Paper

Potent stimulation of vascular endothelial cell growth by differentiated 3T3 adipocytes

Proceedings of the National Academy of Sciences of the United States of America
J J CastellotB M Spiegelman

Abstract

3T3 cells that have undergone adipose differentiation in vitro secrete into the culture medium a potent growth stimulatory activity for bovine aortic endothelial cells. When medium containing 2% fetal calf serum, which does not support significant endothelial cell growth, is conditioned by 3T3-F442A adipocytes, the endothelial cells grow rapidly (doubling time, 24 hr) at a rate equal to the growth rate in 20% fetal calf serum. The potency of the conditioned medium is further shown by the fact that it can be diluted 1:5 with little apparent loss of activity and shows a half-maximal stimulation at 10 microliter/ml. Serum is not required for either the secretion of this mitogen by the adipocytes or its action on the endothelial cells, as shown by the fact that the latter are stimulated to divide in serum-free medium conditioned by the adipocytes. The growth stimulatory activity appears to be specific for vascular endothelial cells in that no other cell type examined, including vascular smooth muscle cells and pericytes, are significantly stimulated by medium conditioned by 3T3-F442A cells. Similarly, medium conditioned by no other cell type examined has more than 10% of the activity of medium conditioned by the adipocytes. The spe...Continue Reading

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