Potent tumor-specific immunity induced by an in vivo heat shock protein-suicide gene-based tumor vaccine

Cancer Research
Wenhong RenXue F Huang

Abstract

Tumor cells harbor a repertoire of unique, mutated antigens and shared self-antigens but generally are incapable of provoking an effective immune response, likely because of inadequate antigen presentation by professional antigen-presenting cells. Heat shock proteins (HSPs) play important roles in eliciting innate and adaptive immunity by chaperoning peptides for antigen presentation and providing endogenous danger signaling. Although effective in inducing tumor-specific immunity in mice and in some clinical trials, tumor-derived HSPs have many limitations like vaccines, such as the technical difficulty of ex vivo preparation of adequate quantities of HSPs from the resected tumors of individual patients. Here we have developed an in vivo HSP-suicide gene tumor vaccine by generating a recombinant replication-defective adenovirus (Ad-HT) that coexpresses HSP70 and a herpes simplex virus thymidine kinase suicide gene. The combination of HSP70 overexpression in situ and tumor killing by thymidine kinase/ganciclovir treatment, but neither strategy alone, provoked potent systemic antitumor activities after intratumor injection of Ad-HT. Tumor-specific CD4+ and CD8+ T-cell responses were induced by Ad-HT intratumor injection. CD11c+ d...Continue Reading

References

Feb 21, 1990·Journal of the National Cancer Institute·F L Moolten, J M Wells
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·H UdonoP K Srivastava
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S H ChenS L Woo
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·D L StolerG R Anderson
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R J BinderP K Srivastava
Mar 23, 2001·Nature Immunology·P K Srivastava
Mar 15, 2001·Nature Immunology·H Schild, H G Rammensee
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S SomersanN Bhardwaj
Apr 12, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Brigitte M PützerHelmut Esche
Jul 12, 2002·Cancer Immunology, Immunotherapy : CII·Xiang-Yang WangJohn R Subjeck
Oct 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Filiberto BelliGiorgio Parmiani
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elfriede NoessnerRolf D Issels
Dec 4, 2002·The Journal of Experimental Medicine·Julie C Baker-LePainChristopher V Nicchitta
Dec 24, 2002·Current Opinion in Immunology·Julie C Baker-LePainChristopher V Nicchitta
Jan 23, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·V MilaniR D Issels

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Citations

Jul 29, 2011·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ya-Ju HsiehRen-Shyan Liu
Sep 3, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Makiya NishikawaYoshinobu Takakura
Oct 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiao-Tong SongStephen Gottschalk
Jan 6, 2011·The Journal of Clinical Investigation·Bangxing HongSi-Yi Chen
Jul 30, 2005·Expert Opinion on Biological Therapy·Delia J NelsonRobbert van der Most
Sep 20, 2006·Expert Opinion on Therapeutic Targets·Hong Pyo KimAugustine M K Choi
Sep 18, 2018·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Ken AndoTakashi Nakano
Feb 9, 2018·JCI Insight·Danhong WangHu Chen
Apr 2, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·H LiQ Guo

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