PMID: 7542902Jun 1, 1995Paper

Potential activities of androgen metabolizing enzymes in human prostate

The Journal of Steroid Biochemistry and Molecular Biology
M KriegS Tunn

Abstract

The entire androgen metabolism of the human prostate is an integral part of the DHT mediated cellular processes, which eventually give rise to the androgen responsiveness of the prostate. Therefore, the potential activities of various androgen metabolizing enzymes were studied. Moreover, the impact of aging on the androgen metabolism and the inhibition of 5 alpha-reductase by finasteride were studied. In epithelium (E) and stroma (S) of normal (NPR) and hyperplastic human prostate (BPH), for each enzyme being involved in the conversion either of testosterone via DHT, 3 alpha- and 3 beta-diol to the C19O3-triols or from testosterone to androstenedione and vice versa, the amount (Vmax) and Michaelis constant (Km) were determined by Lineweaver-Burk plots. Furthermore, Vmax/Km quotients were calculated, which served as an index for the potential enzyme activity. 17 enzymes showed a mean Vmax/Km > or = 0.10. The top four were the 5 alpha-reductases in E and S of NPR and BPH. Among those, the highest activity was found in E of NPR (1.6 +/- 0.2). Moreover, in E a significant age-dependent decrease of 5 alpha-reductase activity occurred, whereas in stroma rather constant activities were found over the whole age range. Similar age-depen...Continue Reading

References

Jan 1, 1992·The Journal of Clinical Investigation·E P JenkinsD W Russell
Dec 1, 1990·The Journal of Clinical Endocrinology and Metabolism·J Geller
Feb 1, 1990·Journal of Steroid Biochemistry·K Nahoul, M Roger
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Jul 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·S TunnM Krieg

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Citations

Nov 2, 1999·The Prostate·W E Farnsworth
Jun 5, 1999·European Journal of Pharmacology·Z Y SunZ H Tu
Oct 21, 1998·The Journal of Steroid Biochemistry and Molecular Biology·H Weisser, M Krieg
Jan 14, 2011·Nature Reviews. Urology·Clement K M Ho, Fouad K Habib
Oct 8, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Chao WangJu Zhang
Feb 9, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·Marisa CabezaEugene Bratoeff
May 11, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Frank GitonJean Fiet
Feb 5, 2002·Chemical & Pharmaceutical Bulletin·Elena RamirezAlfonso Lira
Nov 16, 2004·Andrologia·D SchultheissU Jonas
Oct 31, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Eugene BratoeffMarisa Cabeza

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