Potential and limitations of X-Ray micro-computed tomography in arthropod neuroanatomy: a methodological and comparative survey

The Journal of Comparative Neurology
Andy SombkeSteffen Harzsch

Abstract

Classical histology or immunohistochemistry combined with fluorescence or confocal laser scanning microscopy are common techniques in arthropod neuroanatomy, and these methods often require time-consuming and difficult dissections and sample preparations. Moreover, these methods are prone to artifacts due to compression and distortion of tissues, which often result in information loss and especially affect the spatial relationships of the examined parts of the nervous system in their natural anatomical context. Noninvasive approaches such as X-ray micro-computed tomography (micro-CT) can overcome such limitations and have been shown to be a valuable tool for understanding and visualizing internal anatomy and structural complexity. Nevertheless, knowledge about the potential of this method for analyzing the anatomy and organization of nervous systems, especially of taxa with smaller body size (e.g., many arthropods), is limited. This study set out to analyze the brains of selected arthropods with micro-CT, and to compare these results with available histological and immunohistochemical data. Specifically, we explored the influence of different sample preparation procedures. Our study shows that micro-CT is highly suitable for an...Continue Reading

References

Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C H FoxP P Roller
Aug 16, 2000·Cell·L B VosshallR Axel
Feb 13, 2002·Current Biology : CB·Karlheinz ReinMartin Heisenberg
Apr 12, 2002·Journal of Morphology·Thomas HörnschemeyerFreek Pasop
Mar 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Swidbert R Ott, Maurice R Elphick
Aug 4, 2005·Journal of Electron Microscopy·Akira TanisakoHideyuki Yoshimura
Sep 22, 2005·The Journal of Comparative Neurology·Robert BrandtRandolf Menzel
May 10, 2006·PLoS Genetics·John T JohnsonCharles Keller
Apr 17, 2007·Journal of Synchrotron Radiation·Ryuta MizutaniYoshio Suzuki
Nov 29, 2007·The Journal of Comparative Neurology·Christina ZubeWolfgang Rössler
Apr 11, 2008·Journal of Neuroscience Methods·Willi RibiShaowu Zhang
May 9, 2008·Journal of Neuroscience Methods·Alex de CrespignyHelen E D'Arceuil
May 28, 2008·Cell and Tissue Research·Angela E KurylasUwe Homberg
Jun 17, 2008·Journal of Synchrotron Radiation·Ryuta MizutaniYoshio Suzuki
Jun 17, 2008·Arthropod Structure & Development·Rolf G BeutelMichael F Whiting
Sep 27, 2008·Tissue Engineering. Part C, Methods·Ryuta MizutaniYoshio Suzuki
Feb 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brian D Metscher
Sep 5, 2009·The Journal of Comparative Neurology·Basil El JundiJoachim Schachtner
Mar 27, 2010·Frontiers in Systems Neuroscience·Wolf HuetterothJoachim Schachtner
Nov 11, 2010·Frontiers in Zoology·Stefan RichterSteffen Harzsch
Feb 22, 2011·Arthropod Structure & Development·Nicholas J Strausfeld, David R Andrew
May 3, 2011·Nature Methods·Hanchuan PengJulie H Simpson
Apr 1, 2006·Integrative and Comparative Biology·Steffen Harzsch
Nov 1, 2011·Micron : the International Research and Review Journal for Microscopy·Ryuta Mizutani, Yoshio Suzuki
May 9, 2012·Journal of Microbiological Methods·Nur Hazlin Hazrin-Chong, Mike Manefield
Jun 13, 2012·Nature Methods·Stephan SaalfeldPavel Tomancak
Oct 20, 2012·Arthropod Structure & Development·Frank FriedrichRolf G Beutel
Feb 21, 2013·Journal of Insect Science·Mark K GrecoMarc O Schäfer

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Citations

Dec 22, 2016·Scientific Reports·Peter SwartHolger G Krapp
Oct 12, 2016·Scientific Reports·Ryuta MizutaniKentaro Uesugi
Apr 22, 2017·Scientific Reports·Jay A StafstromEileen A Hebets
Feb 23, 2018·Journal of Microscopy·Henry JahnJörg U Hammel
Jun 10, 2016·Journal of Cell Science·Tom ShearerSarah H Cartmell
Jun 7, 2018·Integrative and Comparative Biology·Lauren Sumner-Rooney
Feb 24, 2018·Nature Communications·Andrew A WalkerGlenn F King
Jan 22, 2020·The Journal of Comparative Neurology·Philip O M SteinhoffAndy Sombke
May 10, 2020·Journal of Morphology·Mariela A Oviedo-DiegoAlfredo V Peretti
Nov 10, 2018·Scientific Reports·Venera WeinhardtFelix Loosli
Oct 30, 2019·Scientific Reports·Lauren Sumner-RooneySuzanne T Williams
Sep 28, 2018·Scientific Reports·Diego CastejónGuillermo Guerao
May 3, 2019·Journal of Biological Engineering·Qinfeng WuJeremiah J Zartman
Nov 15, 2019·Development·Todd A SchoborgNasser M Rusan
Mar 5, 2020·Scientific Reports·Simone FerstlFranz Pfeiffer
Dec 24, 2018·Frontiers in Zoology·Tobias Lehmann, Roland R Melzer
Sep 1, 2020·Bone·M P Akhter, R R Recker
Feb 14, 2021·Communications Biology·Marco GinzelDietrich Kluth

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Methods Mentioned

BETA
X-ray
dissection
chemical fixation
scanning
transmission electron microscopy
fluorescence microscopy
light microscopy
fluorescence

Software Mentioned

SAP VEA
XMReconstructor
VRML Export
Amira
Fiji
voltex
Adobe Acrobat
Deep Exploration

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