Abstract
Medicinal plants are potential sources for a wide range of complex compounds with probable anticancer activity. Ephedra foeminea Forssk. (E. foeminea), a medicinal plant found in the Eastern Mediterranean, has recently been gaining popularity as a cancer remedy; there is, however, a paucity of empirical evidence supporting this claim. In this study, the effect of E. foeminea ethyl acetate, ethanol, and water crude extracts on viability, migratory ability, and the steady-state mRNA levels of genes involved in these processes was, respectively, examined using MTT assay, wound healing assay, and reverse transcriptase PCR (RT-PCR). The study concludes that all extracts significantly reduce human osteosarcoma U2OS percentage viability in a dose- and time-dependent manner, with varying potencies. The least half-maximal inhibitory concentration (IC50) was observed in the water extract after 48 h incubation (30.761 ± 1.4 μg/mL) followed by the ethyl acetate extract after 72 h incubation (80.35 ± 1.233 μg/mL) and finally the ethanol extract after 48 h incubation (97.499 ± 1.188 μg/mL). Ethanol extract significantly reduced U2OS percentage wound closure. On the other hand, both ethanol and water extracts considerably reduced the steady-s...Continue Reading
References
May 22, 1986·Journal of Immunological Methods·F Denizot, R Lang
Jan 1, 1993·Cancer Treatment and Research·S M BlaneyJ L Grem
Sep 20, 2001·Current Medicinal Chemistry·A K MukherjeeA C Ghosh
Apr 16, 2002·British Journal of Cancer·E L KornR Simon
Apr 30, 2003·Cell Death and Differentiation·M Oren
Mar 31, 2004·The Lancet Oncology·Frank EntschladenKurt S Zaenker
Apr 27, 2004·Oncogene·Lixin SunAntonino Passaniti
Dec 31, 2004·The American Journal of Psychiatry·Margaret MaglionePaul G Shekelle
Jun 10, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·David HuiRaymond Lai
Aug 10, 2006·International Orthopaedics·Dorothe Carrle, Stefan S Bielack
Dec 14, 2006·Cancer Metastasis Reviews·J PratapG S Stein
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Sep 12, 2007·Cancer Cell International·Khaldoun Al-RomaihJeremy A Squire
Apr 22, 2009·Molecular and Cellular Biology·Laurie ReyJean-Sébastien Hoffmann
Sep 26, 2009·Journal of Chemical Biology·Heino Prinz
Jan 1, 2009·Expert Opinion on Medical Diagnostics·Colin Kong, Marc F Hansen
Mar 18, 2011·Malaria Journal·Philippe RasoanaivoBen Gilbert
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 6, 2012·Journal of Cellular Physiology·Claudia M J LuceroMario A Galindo
Jun 19, 2013·Nucleic Acids Research·Daniel MenendezMichael A Resnick
Jun 3, 2014·Molecular Cancer·Ingvild HaalandBjørn Tore Gjertsen
Aug 13, 2014·BMC Urology·Celeste De MonteCosimo De Nunzio
Nov 5, 2014·Advanced Pharmaceutical Bulletin·Elham SafarzadehBehzad Baradaran
Mar 3, 2015·Oncotarget·Purificacion Estevez-GarciaRocio Garcia-Carbonero
Aug 22, 2015·Bosnian Journal of Basic Medical Sciences·Saida Ibragic, Emin Sofić
Apr 4, 2016·Seminars in Cancer Biology·Muthu K ShanmugamGautam Sethi
May 6, 2016·Scientific Reports·Shao-Xing DaiJing-Fei Huang
May 9, 2016·Journal of Cancer Research and Clinical Oncology·Eran Ben-AryeNoah Samuels
Jan 19, 2017·Oncotarget·Zhixiang ZhaoDong M Shin
Apr 4, 2017·Journal of Cellular Biochemistry·Oscar A VegaMario A Galindo
Apr 26, 2018·Chinese Medicine·Qing-Wen ZhangWen-Cai Ye
Apr 16, 2019·Oxidative Medicine and Cellular Longevity·Anderson Junger Teodoro