Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections

Viruses
Yuan-Chuan ChenFenyong Liu

Abstract

The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus. CRISPR/Cas9-directed mutagenesis can introduce similar types of mutations to the viral genome as can bacterial artificial chromosome recombination engineering, which maintains and reconstitutes the viral genome successfully. The cleavage mediated by CRISPR/Cas9 enables the manipulation of disease-associated viral strains with unprecedented efficiency and precision. Additionally, current therapies for herpesvirus productive and latent infections are limited in efficacy and cannot eradicate viruses. CRISPR/Cas9 is potentially adapted for antiviral treatment by specifically targeting viral genomes during latent infections. This review, which focuses on recently published progress, suggests that the CRISPR/Cas9 system is not only a useful tool for basic virology research, but also a promising strategy for the control and prevention of herpesvirus latent infections.

References

Jun 20, 2008·Clinical Orthopaedics and Related Research·Wei GuoHui Zhao
Jan 9, 2010·Science·Philippe Horvath, Rodolphe Barrangou
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yong BaiFenyong Liu
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Jan 31, 2013·Nature Biotechnology·Woong Y HwangJ Keith Joung
Mar 6, 2013·Nucleic Acids Research·James E DiCarloGeorge M Church
Mar 8, 2013·Nature·Emmanuelle Charpentier, Jennifer A Doudna
Jul 3, 2013·Nature Methods·Ari E FriedlandJohn A Calarco
Aug 20, 2014·Molecular Therapy. Nucleic Acids·Su-Ru LinPei-Jer Chen
Aug 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianbin Wang, Stephen R Quake
Sep 10, 2014·Journal of Cellular Physiology·Swadesh K DasPaul B Fisher
Nov 14, 2015·Science·Spencer C KnightRobert Tjian
Apr 12, 2016·Scientific Reports·Pamela C RoehmKamel Khalili
May 27, 2016·Journal of Virology·Craig J BierleMark R Schleiss
Jul 1, 2016·PLoS Pathogens·Ferdy R van DiemenRobert Jan Lebbink
Oct 14, 2016·Nature·Martin P StewartKlavs F Jensen
Oct 21, 2016·Drug Development Research·Walter H MoosKosta Steliou
Nov 20, 2016·Cellular Microbiology·Ferdy R van Diemen, Robert Jan Lebbink
Apr 4, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chaoran YinWenhui Hu
Apr 5, 2017·Annual Review of Biophysics·Fuguo Jiang, Jennifer A Doudna
Jun 13, 2017·Current Opinion in Microbiology·Eugene V KooninFeng Zhang
Nov 2, 2017·Einstein·Giulliana Augusta Rangel Gonçalves, Raquel de Melo Alves Paiva
Nov 12, 2017·Nature Communications·Mikihiro ShibataOsamu Nureki

❮ Previous
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Citations

Nov 18, 2018·F1000Research·Richard Whitley, Joel Baines
Sep 13, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Anna BellizziHassen S Wollebo
Dec 15, 2019·Virchows Archiv : an International Journal of Pathology·Masashi FukayamaAtsushi Kaneda
Sep 29, 2020·Nature Communications·Marius Walter, Eric Verdin
Sep 5, 2019·International Journal of Molecular Sciences·Shao-Cheng WangChing-Ming Cheng
Sep 10, 2020·International Journal of Molecular Sciences·Shao-Cheng WangChing-Ming Cheng
Nov 6, 2018·Frontiers in Microbiology·Francisco J IbáñezPablo A González
Feb 23, 2020·International Journal of Molecular Sciences·Shao-Cheng WangYuan-Chuan Chen
Mar 3, 2020·Frontiers in Microbiology·Diana M ÁlvarezPablo A González
Aug 20, 2020·Nature Communications·Martine AubertKeith R Jerome
Feb 18, 2020·Brain Structure & Function·Jose L Lanciego, Floris G Wouterlood
Nov 27, 2020·Current Opinion in Virology·Alejandro MatíaRafael Blasco
Jun 4, 2021·The AAPS Journal·Amanda Catalina Ramirez-Phillips, Dexi Liu
Jun 3, 2021·Viruses·Miroslava ŠudomováSherif T S Hassan
Aug 13, 2021·Expert Opinion on Drug Discovery·Chih-Yeu Fang, Chia-Chyi Liu
Jul 10, 2019·ACS Applied Materials & Interfaces·Magdalena PachotaMaria Nowakowska

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Methods Mentioned

BETA
transfection
PCR
genotyping
transfections

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