Potential biomarkers and targets in reversibility of pulmonary arterial hypertension secondary to congenital heart disease: an explorative study

Pulmonary Circulation
Li HuangJianguo He

Abstract

Whether pulmonary arterial hypertension (PAH) is reversible in congenital heart disease (CHD) is important for the operability of CHD. However, little is known about that. Our research was aimed at exploring novel biomarkers and targets in the reversibility of CHD-PAH. CHD-PAH patients diagnosed with right heart catheterization (RHC) were enrolled (n = 14). Lung biopsy was performed during the repair surgery. After one year follow-up, mean pulmonary arterial pressures (mPAP) were evaluated by RHC to determine the diagnosis of reversible (mPAP < 25 mmHg, n = 10) and irreversible (mPAP ≥ 25 mmHg, n = 4) PAH. Harvested normal lung tissues (n = 6) were included as the control group. Pulmonary arteriole lesions were identified by pathological grading in tissue staining. iTRAQ-labelled mass-spectrometry analysis followed by immunohistochemistry and western blot was used to explore the most meaningful differential proteins. For enrolled patients, the histopathological grading of pulmonary vascular lesions in reversible CHD-PAH patients was all at grades 0-II while grades III-IV were shown only in irreversible CHD-PAH patients. Proteomic analysis identified 85 upregulated and 75 downregulated proteins, including cytoskeletal proteins a...Continue Reading

References

Mar 17, 2001·Nature Reviews. Molecular Cell Biology·T P StosselS S Shapiro
May 2, 2002·Journal of Cardiovascular Risk·Xing Li WangDavid E L Wilcken
Jun 14, 2002·The Journal of Thoracic and Cardiovascular Surgery·L LindbergC Jonmarker
Apr 12, 2003·Developmental Cell·Thomas P Stossel, John H Hartwig
Dec 22, 2006·International Journal of Cardiology·M G J DuffelsB J M Mulder
May 2, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hemal H PatelPaul A Insel
Jan 12, 2010·Trends in Cell Biology·Alex-Xianghua ZhouLevent M Akyürek
Aug 15, 2012·Experimental Biology and Medicine·Jing HuangRajamma Mathew
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiaowei ZhengLorenz Poellinger

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Citations

May 16, 2018·Circulation Journal : Official Journal of the Japanese Circulation Society·Susumu HosokawaShozaburo Doi
Oct 30, 2020·Frontiers in Pharmacology·Xue BaiYu-Ping Tang
Jun 3, 2021·Antioxidants·Daria S Kostyunina, Paul McLoughlin
Nov 23, 2021·Frontiers in Pharmacology·Yang-Yang HeZhi-Yan Han

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Methods Mentioned

BETA
electrophoresis
biopsy

Software Mentioned

MetaCore
MetaCoreTM
GraphPad Prism
SPSS
DAVID
ProteinPilot
Quantity One

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