Potential contribution of a voltage-activated proton conductance to acid extrusion from rat hippocampal neurons

Neuroscience
Y M ChengJ Church

Abstract

We examined the potential contribution of a voltage-gated proton conductance (gH+) to acid extrusion from cultured postnatal rat hippocampal neurons. In neurons loaded with Ca2+- and/or pH-sensitive fluorophores, transient exposures to 25-139.5 mM external K+ (K+o) or 20 microM veratridine in the presence of 2 mM Ca2+o (extracellular pH (pHo) constant at 7.35) caused reversible increases and decreases in intracellular free calcium concentration ([Ca2+]i) and intracellular pH (pHi), respectively. In contrast, under external Ca2+-free conditions, the same stimuli failed to affect [Ca2+]i but caused an increase in pHi, the magnitude of which was related to the [K+]o applied and the change in membrane potential. Consistent with the properties of gH+s in other cell types, the magnitude of the rise in pHi observed in the absence of external Ca2+ was not affected by the removal of external Na+ but was sensitive to external Zn2+ and temperature and was dependent on the measured transmembrane pH gradient (DeltapHmemb). Increasing DeltapH(memb) by pretreatment with carbonylcyanide-p-trifluoromethoxyphenylhydrazone augmented both the high-[K+]o-evoked rise in pHi and the Zn2+-sensitive component of the rise in pHi, suggestive of increased...Continue Reading

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Citations

May 9, 2008·Cellular and Molecular Life Sciences : CMLS·T E DeCoursey
Jan 12, 2012·Neuroscience Bulletin·Wei-Zheng Zeng, Tian-Le Xu
Mar 6, 2012·Nature Neuroscience·Long-Jun WuDavid E Clapham
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May 13, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nataliya SvicharMitchell Chesler
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Mar 17, 2009·Biochemical and Biophysical Research Communications·Yoshifumi OkochiYasushi Okamura
Jan 17, 2020·Chemistry : a European Journal·Alejandro Méndez-ArdoyJavier Montenegro
Jan 28, 2014·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Long-Jun Wu
Oct 17, 2009·Chemical Reviews·Junyan Han, Kevin Burgess

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