Potential electron mediators to extract electron energies of RBC glycolysis for prolonged in vivo functional lifetime of hemoglobin vesicles

Bioconjugate Chemistry
Karin KettisenHiromi Sakai

Abstract

Developing a functional blood substitute as an alternative to donated blood for clinical use is believed to relieve present and future blood shortages, and to reduce the risks of infection and blood type mismatching. Hemoglobin vesicle (HbV) encapsulates a purified and concentrated human-derived Hb solution in a phospholipid vesicle (liposome). The in vivo safety and efficacy of HbV as a transfusion alternative have been clarified. Auto-oxidation of ferrous Hb in HbV gradually increases the level of ferric methemoglobin (metHb) and impairs the oxygen transport capabilities. The extension of the functional half-life of HbV has recently been proposed using an electron mediator, methylene blue (MB), which acts as a shuttle between red blood cells (RBC) and HbV. MB transfers electron energies of NAD(P)H, produced by RBC glycolysis, to metHb in HbV. Work presented here focuses on screening of 15 potential electron mediators, with appropriate redox potential and water solubility, for electron transfer from RBC to HbV. The results are assessed with regard to the chemical properties of the candidates. The compounds examined in this study were dimethyl methylene blue (DMB), methylene green, azure A, azure B, azure C, toluidine blue (TDB...Continue Reading

References

Oct 26, 1999·Annals of Emergency Medicine·R O WrightA D Woolf
Dec 19, 2000·International Journal of Antimicrobial Agents·M Wainwright
Nov 1, 2001·Biochimica Et Biophysica Acta·J M MayJ D Morrow
Feb 20, 2004·American Journal of Physiology. Cell Physiology·James M MayCharles E Cobb
Jan 31, 2009·Artificial Organs·Hiromi SakaiEishun Tsuchida
Feb 12, 2009·Bioconjugate Chemistry·Eishun TsuchidaKoichi Kobayashi
Mar 20, 1930·The Journal of General Physiology·E S Barron, L A Hoffman
Mar 14, 2012·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Thomas M S Chang
Mar 19, 2014·Trends in Biotechnology·Abdu I Alayash

❮ Previous
Next ❯

Citations

Apr 18, 2017·Journal of Pharmaceutical Sciences·Kazuaki TaguchiMasaki Otagiri
Aug 31, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Lilla MacsaiZsolt Datki
Feb 20, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Prashanth Kumar KoochanaRabindra K Behera
Jul 30, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Hiromi Sakai, Cassandra Leong
Feb 19, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Prashanth Kumar KoochanaRabindra K Behera

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Group Incompatibility

Blood group incompatibility between donor and recipient has been associated with poor transplant outcomes. Discover the latest research on blood group icompatibility here.