Potential gene regulatory role for cyclin D3 in muscle cells

Journal of Biosciences
Fathima Athar, Veena K Parnaik

Abstract

Cyclin D3 is important for muscle development and regeneration, and is involved in post-mitotic arrest of muscle cells. Cyclin D3 also has cell-cycle-independent functions such as regulation of specific genes in other tissues. Ectopic expression of cyclin D3 in myoblasts, where it is normally undetectable, promotes muscle gene expression and faster differentiation kinetics upon serum depletion. In the present study, we investigated the mechanistic role of cyclin D3 in muscle gene regulation. We initially showed by mutational analysis that a stable and functional cyclin D3 was required for promoting muscle differentiation. Using chromatin immunoprecipitation assays, we demonstrated that expression of cyclin D3 in undifferentiated myoblasts altered histone epigenetic marks at promoters of muscle-specific genes like MyoD, Pax7, myogenin and muscle creatine kinase but not non-muscle genes. Cyclin D3 expression also reduced the mRNA levels of certain epigenetic modifier genes. Our data suggest that epigenetic modulation of muscle-specific genes in cyclin-D3-expressing myoblasts may be responsible for faster differentiation kinetics upon serum depletion. Our results have implications for a regulatory role for cyclin D3 in muscle-spec...Continue Reading

References

Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·P W HindsR A Weinberg
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·C McMahonM E Ewen
Sep 21, 2001·Molecular and Cellular Biology·N A TimchenkoL T Timchenko
Jan 7, 2004·Cancer Cell·Ewa SicinskaPiotr Sicinski
Jun 16, 2004·Current Opinion in Genetics & Development·Ru Cao, Yi Zhang
Sep 11, 2004·Biochemical and Biophysical Research Communications·Wenjin LiuJianxin Gu
Nov 3, 2004·Genes & Development·Giuseppina CarettiVittorio Sartorelli
Feb 11, 2005·Molecular Biology of the Cell·Indumathi Mariappan, Veena K Parnaik
Aug 18, 2005·Biochemical and Biophysical Research Communications·Yongzhi JianJianxin Gu
Sep 13, 2005·Journal of Cellular Physiology·Daniela Palacios, Pier Lorenzo Puri
Nov 2, 2005·Molecular and Cellular Biology·David A SarrufLluis Fajas
Sep 29, 2006·Journal of Biosciences·Veena K Parnaik, Kaliyaperumal Manju
Feb 27, 2007·Biochemical and Biophysical Research Communications·Indumathi MariappanVeena K Parnaik
Aug 22, 2007·Molecular and Cellular Biology·Francesca De SantaMaurizia Caruso
Nov 26, 2008·Molecular Cell·Raphael MargueronDanny Reinberg
Jul 4, 2009·BMC Molecular Biology·Jens Stern-StraeterUlrich R Goessler
Dec 1, 2009·Biochemical and Biophysical Research Communications·Sanna Barrand, Philippe Collas
Dec 17, 2009·Epigenetics : Official Journal of the DNA Methylation Society·Eusebio PerdigueroPura Muñoz-Cánoves
Mar 20, 2010·The EMBO Journal·Shayesta SeenundunFrancis Jeffrey Dilworth
May 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Patrik AspBrian David Dynlacht
Aug 24, 2011·The Journal of Cell Biology·Yazhong TaoDa-Zhi Wang
Sep 8, 2011·Journal of Cellular Biochemistry·Ritika Gurung, Veena K Parnaik
Jan 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Belinda Mei Tze LingReshma Taneja
Nov 20, 2012·The Journal of Clinical Investigation·Karlie JonesLubov T Timchenko

❮ Previous
Next ❯

Citations

Jul 3, 2017·Journal of Cell Science·Nana NaetarRoland Foisner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.