Potential genes associated with survival of Acinetobacter baumannii under ciprofloxacin stress.

Microbes and Infection
Shruti KashyapNeena Capalash

Abstract

Acinetobacter baumannii is an opportunistic pathogen that has acquired resistance to all available drugs. The rise in multi-drug resistance in A. baumannii has been exacerbated by its ability to tolerate antibiotics due to the persister cells, which are phenotypic variants of normal cells that can survive various stress conditions, resulting in chronicity of infection. In the present study we observed that A. baumannii formed persister cells against lethal concentration of ciprofloxacin in exponential phase. The transcriptome of A. baumannii was analyzed after exposure to high concentration of ciprofloxacin (50X MIC) to determine the possible mechanisms of survival. Transcriptome analysis showed differential expression of 146 genes, of which 101 were up-regulated and 45 were down-regulated under ciprofloxacin stress. Differentially expressed genes that might be important for persistence against ciprofloxacin were involved in DNA repair, phenylacetic acid degradation, leucine catabolism, HicAB toxin-antitoxin system and ROS response (iron-sulfur clusters, hemerythrin-like metal binding and Kdp). recA, umuD and ddrR genes involved in SOS response were also up-regulated. Knockout of umuD showed significant decrease in persister ce...Continue Reading

References

Oct 1, 1977·Journal of General Microbiology·T D BrownH L Kornberg
Apr 14, 2004·Journal of Applied Microbiology·H C BeckF R Lauritsen
Dec 18, 2007·FEMS Microbiology Letters·Christopher E FrenchF Bruce Ward
Jan 20, 2009·Applied and Environmental Microbiology·Barbara SchmidTaurai Tasara
Dec 17, 2009·PLoS Genetics·Tobias DörrMarin Vulić
Jan 16, 2010·Journal of Microbiology and Biotechnology·Seol-Hee KimYou-Hee Cho
May 1, 2010·Journal of Cellular Physiology·Jun WuJianping Xie
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·R TeufelG Fuchs
Apr 5, 2013·PLoS Pathogens·Zoë N FreemanNicholas R Waterfield
Apr 30, 2013·Journal of Bacteriology·Marat R SadykovKenneth W Bayles
Oct 15, 2013·Journal of Bacteriology·Jesús ArandaGermán Bou
Oct 30, 2013·FEMS Microbiology Letters·Kevin T MilitelloRobert D Simon
Jan 17, 2014·The Journal of Infectious Diseases·Gustavo M CerqueiraAnton Y Peleg
Feb 8, 2014·The Biochemical Journal·Aaron ButtRichard W Titball
Jan 15, 2015·Microbiology Spectrum·George A JacobyDavid C Hooper
Jul 28, 2016·Cold Spring Harbor Perspectives in Medicine·David C Hooper, George A Jacoby
Aug 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Md Saruar BhuiyanAnton Y Peleg
Dec 8, 2016·The Journal of Infectious Diseases·Reham SamirAhmed S Attia
Mar 24, 2017·FEMS Microbiology Reviews·Bram Van den BerghJan Michiels
Jun 14, 2017·Journal of Applied Microbiology·J LemireV D Appanna
Nov 15, 2017·Antimicrobial Agents and Chemotherapy·Natalia BlancoUNKNOWN the CDC Epicenters Program
Feb 6, 2018·Molecular Cell·Alexander HarmsKenn Gerdes
Jun 15, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rashad AlkasirBaoli Zhu
Jul 25, 2018·Scientific Reports·Amanjot KaurNeena Capalash
Nov 14, 2018·International Journal of Antimicrobial Agents·Marion NicolEmmanuelle Dé
Nov 30, 2018·Annual Review of Physiology·Michael NeinastZoltan Arany
Dec 7, 2018·Current Opinion in Infectious Diseases·José Garnacho-Montero, Jean-François Timsit
Apr 14, 2019·Nature Reviews. Microbiology·Nathalie Q BalabanAnnelies Zinkernagel
Aug 4, 2019·Nature Biotechnology·Daehwan KimSteven L Salzberg
Sep 11, 2019·Annual Review of Microbiology·Bridget GollanSophie Helaine

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