Potential immune modularly role of glycine in oral gingival inflammation

Clinical & Developmental Immunology
Teresa SchaumannAndreas Jäger

Abstract

Gingival epithelial cells (GECs) represent a physical barrier against bacteria and are involved in the processes of innate immunity. Recently, an anti-inflammatory and immune-modulatory effect of the amino acid glycine has been demonstrated. However, there is only little information about the immune-modulatory effects of glycine in oral tissues. This study aimed to investigate the existence and role of the glycine receptor in gingival tissue analyzing tissues/cells from extracted human molars via immunohistochemical analysis. In vitro, GECs were challenged by inflammatory conditions with IL-1 β alone or in combination with glycine and analyzed for cytokine expression of IL6/IL8 via real-time PCR. On protein level, the effect of nuclear translocalization of NF κ B protein p65 was analyzed using immunofluorescence analysis. A distinct proof of the GlyR in oral gingival tissue and keratinocytes could be demonstrated. Isolated challenge of the keratinocytes with IL-1 β as well as with glycine resulted in an upregulation of IL6 and IL8 mRNA expression and activation of NF κ B pathway. The presence of glycine in combination with the inflammatory stimulus led to a significant decrease in inflammatory parameters. These results indicate...Continue Reading

References

Jun 1, 1990·The Journal of Investigative Dermatology·J AnselS Hefeneider
Feb 1, 1996·The European Journal of Neuroscience·C BechadeA Triller
Nov 1, 1996·Biomaterials·S L GoodmanR M Albrecht
Jan 1, 1997·Pharmacology & Therapeutics·S RajendraP R Schofield
Mar 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A SpittlerE Roth
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·R F StachlewitzR G Thurman
Oct 18, 2000·Journal of Clinical Periodontology·A RawlinsonA L Fairclough
Nov 15, 2000·Journal of Dental Research·J SandrosP N Papapanou
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·M D WheelerR G Thurman
Sep 12, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Matthias FrohMichael D Wheeler
Sep 19, 2002·Periodontology 2000·Beverly A Dale
Feb 18, 2003·Current Opinion in Clinical Nutrition and Metabolic Care·Zhi ZhongJohn J Lemasters
Apr 29, 2003·Cephalalgia : an International Journal of Headache·M E NobreM Dominici
Aug 5, 2003·Liver International : Official Journal of the International Association for the Study of the Liver·Rafael BruckRami Hershkoviz
Dec 10, 2003·Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society·Michael S Vaphiades
Apr 28, 2004·Microbes and Infection·Carol M BeltonKenneth T Izutsu
Dec 23, 2004·Journal of Periodontal Research·Torbjørn BreivikPer Kristian Opstad
Aug 13, 2005·Acta Anaesthesiologica Scandinavica·R Y GundersenP K Opstad
Aug 21, 2007·Current Pharmaceutical Design·Timothy I Webb, Joseph W Lynch
Sep 4, 2008·Journal of Periodontology·Kenneth S Kornman
Jan 13, 2011·Clinical Oral Investigations·Marjan NokhbehsaimJames Deschner
Sep 24, 2013·Mediators of Inflammation·Marjan NokhbehsaimJames Deschner

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Citations

Jul 9, 2014·Biochemical and Biophysical Research Communications·Ae Sin LeeWon Kim
Oct 28, 2016·International Journal of Paediatric Dentistry·Maria Teresa Botti Rodrigues SantosRenata Gorjão
Mar 8, 2017·Journal of Proteome Research·Chao-Chao DuXuan-Xian Peng
Mar 25, 2020·Journal of Animal Physiology and Animal Nutrition·Jiayun QiaoYupeng Li
Oct 17, 2018·Nutrients·Odara Maria de Sousa SáEliana Maria Monteiro Caran
Feb 23, 2020·International Journal of Molecular Sciences·Tatiana V TatarinovaJeffrey Davis
Feb 29, 2020·BMC Complementary Medicine and Therapies·Ana M ChangSunethra Rajapakse
Feb 9, 2021·Frontiers in Microbiology·Leanne M CleaverGuy H Carpenter
Jun 12, 2021·Journal of Periodontal & Implant Science·Mengyuan ZhuJinlin Song
Aug 19, 2021·Clinical Oral Investigations·Jens WeusmannAndressa V B Nogueira

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Methods Mentioned

BETA
nuclear translocation
PCR

Software Mentioned

GraphPad Prism
Graph Pad
BLAST
AxioVision

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