Potential Interaction of Plasmodium falciparum Hsp60 and Calpain

The Korean Journal of Parasitology
Seon-Ju YeoHyun Park

Abstract

After invasion of red blood cells, malaria matures within the cell by degrading hemoglobin avidly. For enormous protein breakdown in trophozoite stage, many efficient and ordered proteolysis networks have been postulated and exploited. In this study, a potential interaction of a 60-kDa Plasmodium falciparum (Pf)-heat shock protein (Hsp60) and Pf-calpain, a cysteine protease, was explored. Pf-infected RBC was isolated and the endogenous Pf-Hsp60 and Pf-calpain were determined by western blot analysis and similar antigenicity of GroEL and Pf-Hsp60 was determined with anti-Pf-Hsp60. Potential interaction of Pf-calpain and Pf-Hsp60 was determined by immunoprecipitation and immunofluorescence assay. Mizoribine, a well-known inhibitor of Hsp60, attenuated both Pf-calpain enzyme activity as well as P. falciparum growth. The presented data suggest that the Pf-Hsp60 may function on Pf-calpain in a part of networks during malaria growth.

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Citations

Oct 4, 2018·International Journal of Molecular Sciences·Trinh Thi Thuy TienSeon-Ju Yeo
Nov 30, 2019·International Journal of Molecular Sciences·Tawanda Zininga, Addmore Shonhai
Aug 28, 2021·International Journal of Molecular Sciences·Hien Thi TuongSeon-Ju Yeo

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Methods Mentioned

BETA
immunoprecipitation
FACS
flow cytometry

Software Mentioned

BLASTP
PlasmoDB
GraphPad Prism
BLAST

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