PMID: 9534210Apr 16, 1998Paper

Potential misidentification of Burkholderia pseudomallei by API 20NE

Pathology
Timothy J J InglisL Chor-Kiang

Abstract

Biochemical confirmation of the identity of Burkholderia pseudomallei in Singapore previously relied on the API 20NE panel of tests. After introducing an alternative proprietary biochemical panel, the Microbact 24E (MedVet, Adelaide, Australia), we noted that the API panel identified some presumptive B. pseudomallei isolates as other species. We therefore compared the performance of the API 20NE against the Microbact 24E with 50 distinct clinical isolates of B. pseudomallei, after 24 hours and after five days incubation of primary cultures. The API panel correctly identified 40 isolates. Four results were unacceptable or uninterpretable. Six isolates were misidentified as other species; the commonest being Chromobacterium violaceum. One of these was again identified as C. violaceum by the repeat API panel. Fourteen isolates, including the six misidentified isolates and four isolate pairs from separate sources in four separate patients, were typed using PCR amplification of repetitive extragenic palindromic sequences (REPS). The isolates identified as C. violaceum appeared to have identical REPS patterns, suggesting that some of the errant API results may be due to a single locally prevalent strain of B. pseudomallei. A previous...Continue Reading

References

Apr 1, 1993·Journal of Clinical Pathology·M D SmithT L Pitt
Sep 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T Y TiE H Lee

❮ Previous
Next ❯

Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·P TrivediH V Batra
Feb 16, 2000·Acta Tropica·S SirisinhaY Suputtamongkol
Jan 16, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Valery A AntonovVladimir V Alekseev
Aug 25, 1999·Lancet·H Peltroche-Llacsahuanga, G Haase
Sep 19, 2006·Applied and Environmental Microbiology·Timothy J J Inglis, Jose-Luis Sagripanti
Apr 16, 2005·Clinical Microbiology Reviews·Allen C Cheng, Bart J Currie
Sep 7, 2007·Journal of Clinical Microbiology·Premjit AmornchaiSharon J Peacock
Aug 22, 2008·Journal of Clinical Microbiology·Timothy J J InglisRussell McInnes
Mar 13, 2009·Journal of Clinical Microbiology·Kelly HodgsonRobert Norton
Apr 3, 2001·Journal of Clinical Microbiology·T Schülin, I Steinmetz
Nov 28, 2002·Journal of Clinical Microbiology·Peter LoweRobert Norton
Aug 31, 2002·Journal of Clinical Microbiology·Timothy J J InglisJudy Sampson
Oct 9, 2003·Journal of Clinical Microbiology·Timothy J J InglisBrian J Mee
May 6, 2005·Journal of Clinical Microbiology·Timothy J J InglisGerry Harnett
Jul 31, 2009·Annals of Clinical Microbiology and Antimicrobials·Günther SlesakPaul N Newton
Dec 17, 2005·Emerging Infectious Diseases·Eugene AthanAllen C Cheng
Aug 11, 2011·Journal of Pharmacy & Bioallied Sciences·Duraipandian Thavaselvam, Rajagopalan Vijayaraghavan
Jun 3, 2011·The American Journal of Tropical Medicine and Hygiene·Timothy J J InglisChristopher H Heath
Jan 23, 2014·Emerging Infectious Diseases·Ashley M ZehnderEmily S Beeler
Nov 13, 2004·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Herbert TomasoHeinrich Neubauer
Dec 6, 2007·Wiener klinische Wochenschrift·Herbert TomasoEberhard Straube
Oct 6, 2007·Diagnostic Microbiology and Infectious Disease·Pattarachai KiratisinSrirumpa Kaewdaeng
Nov 5, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L D Sprague, H Neubauer
Jan 28, 2015·Emerging Infectious Diseases·Alex R HoffmasterHenry T Walke
Mar 16, 2005·FEMS Microbiology Letters·Holger Christian ScholzHeinrich Neubauer
Nov 7, 2006·Revista Do Instituto De Medicina Tropical De São Paulo·Adam MerrittGerry Harnett
Mar 21, 2006·Revista Do Instituto De Medicina Tropical De São Paulo·Timothy J J InglisJorge L N Rodriguez
Jul 24, 2012·Journal of Medical Microbiology·Zhiyong ZongTaoyou Zhou
Apr 6, 2006·Journal of Medical Microbiology·Melanie P UlrichRicky L Ulrich
Mar 8, 2019·Journal of Clinical Microbiology·I GassiepR E Norton
Mar 13, 2020·Clinical Microbiology Reviews·I GassiepR Norton
Apr 25, 2015·Experimental Biology and Medicine·Susanna K P LauPatrick C Y Woo
Jan 1, 2014·International Scholarly Research Notices·Damian H GillingCori Pflugradt

❮ Previous
Next ❯

Related Concepts