Potential of atmospheric pressure chemical ionization source in gas chromatography tandem mass spectrometry for the screening of urinary exogenous androgenic anabolic steroids

Analytica Chimica Acta
M RaroOscar J Pozo

Abstract

The atmospheric pressure chemical ionization (APCI) source for gas chromatography-mass spectrometry analysis has been evaluated for the screening of 16 exogenous androgenic anabolic steroids (AAS) in urine. The sample treatment is based on the strategy currently applied in doping control laboratories i.e. enzymatic hydrolysis, liquid-liquid extraction (LLE) and derivatization to form the trimethylsilyl ether-trimethylsilyl enol ether (TMS) derivatives. These TMS derivatives are then analyzed by gas chromatography tandem mass spectrometry using a triple quadrupole instrument (GC-QqQ MS/MS) under selected reaction monitoring (SRM) mode. The APCI promotes soft ionization with very little fragmentation resulting, in most cases, in abundant [M + H](+) or [M + H-2TMSOH](+) ions, which can be chosen as precursor ions for the SRM transitions, improving in this way the selectivity and sensitivity of the method. Specificity of the transitions is also of great relevance, as the presence of endogenous compounds can affect the measurements when using the most abundant ions. The method has been qualitatively validated by spiking six different urine samples at two concentration levels each. Precision was generally satisfactory with RSD values...Continue Reading

References

Jun 3, 2004·Rapid Communications in Mass Spectrometry : RCM·Don H CatlinCaroline K Hatton
Jul 14, 2004·Sports Medicine·Fred Hartgens, Harm Kuipers
Dec 18, 2008·Rapid Communications in Mass Spectrometry : RCM·Maria K ParrWilhelm Schänzer
Jul 20, 2010·Journal of Mass Spectrometry : JMS·T PortolésP Hancock
Apr 21, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Miguel A DelgadilloJosep Marcos
Aug 22, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wim Van GansbekePeter Van Eenoo

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Citations

Aug 6, 2016·Bioanalysis·Francisco Radler de Aquino NetoLuciana Pizzatti
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Glenn LoosDeirdre Cabooter
Dec 23, 2016·Analytical and Bioanalytical Chemistry·Samuel MesihääIlkka Ojanperä
Nov 26, 2016·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Jul 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michele ProttiLaura Mercolini
Jun 23, 2020·Rapid Communications in Mass Spectrometry : RCM·Dayamin Martinez-BritoFrancesco Botrè
Sep 22, 2020·Analytical and Bioanalytical Chemistry·Claudia BressanRosa Ventura
May 19, 2020·Metabolomics : Official Journal of the Metabolomic Society·Mohammad Khodadadi, Morteza Pourfarzam
Jul 14, 2021·Environment International·E ColicinoR J Wright

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