Potential of Bacillus subtilis lipopeptides in anti-cancer I: induction of apoptosis and paraptosis and inhibition of autophagy in K562 cells

AMB Express
Haobin ZhaoQingsheng Huang

Abstract

The lipopeptide iturin from Bacillus subtilis has been found to have a potential inhibitory effect on breast cancer, alveolar adenocarcinoma, renal carcinoma, and colon adenocarcinoma. In this study, the potential of B. subtilis lipopeptides (a mixture of iturin homologues, concentration of 42.75%) to inhibit chronic myelogenous leukemia was evaluated using K562 myelogenous leukemia cells. The results showed that the lipopeptides could completely inhibit the growth of K562 at 100 μM, with an IC50 value of 65.76 μM. The lipopeptides inhibited the profile of K562 via three pathways: (1) induction of paraptosis indicated by the occurrence of cytoplasmic vacuoles, and swelling of the mitochondria and endoplasmic reticulum (ER) without membrane blebbing in the presence of a caspase inhibitor; (2) inhibition of autophagy progress illustrated by the upregulated expression of LCII and P62; and (3) induction of apoptosis by causing ROS burst, and induction of the intrinsic pathway indicated by the upregulated expression of cytochrome c (Cyto-c), bax, and bad, together with downregulated expression of Bcl-2. The ROS-dependent apoptosis and caspase-independent paraptosis were verified using the ROS inhibitor and caspase inhibitor, respect...Continue Reading

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Citations

Mar 5, 2019·Journal of Applied Microbiology·P J NaughtonI M Banat
Sep 16, 2019·Applied Microbiology and Biotechnology·Xixi ZhaoJunling Shi
Aug 20, 2020·Applied Microbiology and Biotechnology·Rongrong YangJunling Shi
Sep 9, 2019·Current Microbiology·Alfredo AmbricoRosaria A Magarelli
Apr 19, 2019·Applied Microbiology and Biotechnology·Shuzhen LeiJunling Shi
Jan 30, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hsin-Yu ChouHui-Min David Wang
May 11, 2021·AMB Express·Haobin ZhaoJunling Shi
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Su-Young HongKye-Man Cho
Feb 18, 2020·ACS Applied Bio Materials·Valeria CastellettoJanne Ruokolainen
Aug 23, 2021·Biochimica Et Biophysica Acta. Biomembranes·Karolina WójtowiczMarcin Łukaszewicz

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Methods Mentioned

BETA
column chromatography
electron microscopy
flow cytometry
Assay

Software Mentioned

GraphPad
GraphPad Prism
GraphPad Primer

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