Potential of DNA methylation in rectal cancer as diagnostic and prognostic biomarkers

British Journal of Cancer
Ruth ExnerGerda Egger

Abstract

Aberrant DNA methylation is more prominent in proximal compared with distal colorectal cancers. Although a number of methylation markers were identified for colon cancer, yet few are available for rectal cancer. DNA methylation differences were assessed by a targeted DNA microarray for 360 marker candidates between 22 fresh frozen rectal tumour samples and 8 controls and validated by microfluidic high-throughput and methylation-sensitive qPCR in fresh frozen and formalin-fixed paraffin-embedded (FFPE) samples, respectively. The CpG island methylator phenotype (CIMP) was assessed by MethyLight in FFPE material from 78 patients with pT2 and pT3 rectal adenocarcinoma. We identified and confirmed two novel three-gene signatures in fresh frozen samples that can distinguish tumours from adjacent tissue as well as from blood with a high sensitivity and specificity of up to 1 and an AUC of 1. In addition, methylation of individual CIMP markers was associated with specific clinical parameters such as tumour stage, therapy or patients' age. Methylation of CDKN2A was a negative prognostic factor for overall survival of patients. The newly defined methylation markers will be suitable for early disease detection and monitoring of rectal can...Continue Reading

References

Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ToyotaJ P Issa
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·S RamaswamyT R Golub
Aug 7, 2002·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Michael D RadmacherRichard Simon
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Barry Iacopetta
Jan 21, 2006·Cancer Research·Hiroyuki MatsubayashiMichael Goggins
Jan 26, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Pavel SovaNancy Kiviat
Oct 27, 2006·The Journal of Molecular Diagnostics : JMD·Shuji OginoCharles S Fuchs
Apr 21, 2007·International Journal of Radiation Oncology, Biology, Physics·Federica BertoliniPier Franco Conte
Nov 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lanlan ShenJean-Pierre J Issa
May 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Michiel F G de MaatDave S B Hoon
Nov 7, 2008·Methods in Molecular Biology·Mihaela CampanPeter W Laird
Jan 20, 2009·Anti-cancer Agents in Medicinal Chemistry·Harlinde De Schutter, Sandra Nuyts
Feb 7, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Chang Do JeeWoo Ho Kim
Aug 12, 2009·Cancer Causes & Control : CCC·Wade S SamowitzMartha L Slattery
Dec 24, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Koichi YagiAtsushi Kaneda
Mar 4, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anna M DahlinBethany R Van Guelpen
Mar 27, 2010·International Journal of Radiation Oncology, Biology, Physics·Barbara HofstetterI Frank Ciernik
May 13, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michiel F G de MaatDave S B Hoon
Feb 25, 2011·Cancer Letters·Verena Jendrossek
Mar 2, 2011·The British Journal of Surgery·K J LeongS P Bach
Jun 11, 2011·Genome Research·Toshinori HinouePeter W Laird
Jun 28, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Christine KuhmannOdilia Popanda
Aug 9, 2011·Cancer Letters·Kenji HibiYutaka Sanada
Mar 28, 2012·Epigenomics·Muriel X G DrahtKim M Smits
Jun 19, 2012·Expert Review of Molecular Diagnostics·Thomas MikeskaAlexander Dobrovic
Jul 14, 2012·International Journal of Colorectal Disease·Giles O ElliottNigel J Belshaw
Jul 20, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Mar 14, 2013·Korean Journal of Pathology·Joo-Shik ShinC Soon Lee
Apr 10, 2013·Epigenomics·Christopher P E Lange, Peter W Laird
Jul 23, 2013·International Journal of Molecular Sciences·Joong-Gook KimJoo Mi Yi
Oct 9, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sara Monteiro-ReisCarmen Jerónimo
Nov 14, 2013·British Journal of Cancer·A BenardD S B Hoon
Nov 22, 2013·International Journal of Cancer. Journal International Du Cancer·Maija R J Kohonen-CorishPierre H Chapuis
Mar 25, 2014·International Journal of Cancer. Journal International Du Cancer·Ann Rita HalvorsenHege Edvardsen

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Citations

Jun 2, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Orsolya GalambBéla Molnár
Apr 11, 2020·Archives of Gynecology and Obstetrics·Shengyun CaiRui Guan
Jul 2, 2020·International Journal of Molecular Sciences·Victoria SarneRita Seeboeck
Nov 30, 2016·Clinical Epigenetics·René A M DirksHendrik Marks
Oct 31, 2018·High-throughput·Maria Eugenia Gallo CantafioMaria Teresa Di Martino
Nov 9, 2018·Frontiers in Pharmacology·Mariam A FouadSamia A Shouman
Jan 29, 2020·World Journal of Gastroenterology : WJG·Chih-Hsiung HsuYu-Ching Chou
Oct 19, 2019·World Journal of Clinical Cases·Yu-Xia Zhan, Guang-Hua Luo

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Methods Mentioned

BETA
PCR

Software Mentioned

R
BRB Array
GraphPad Prism
MethyLight

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