Potential of dried blood self-sampling for cyclosporine c(2) monitoring in transplant outpatients.

Journal of Transplantation
Alexander Benedikt LeichtleGeorg Martin Fiedler

Abstract

Background. Close therapeutic drug monitoring of Cyclosporine (CsA) in transplant outpatients is a favourable procedure to maintain the long-term blood drug levels within their respective narrow therapeutic ranges. Compared to basal levels (C(0)), CsA peak levels (C(2)) are more predictive for transplant rejection. However, the application of C(2) levels is hampered by the precise time of blood sampling and the need of qualified personnel. Therefore, we evaluated a new C(2) self-obtained blood sampling in transplant outpatients using dried capillary and venous blood samples and compared the CsA levels, stability, and clinical practicability of the different procedures. Methods. 55 solid organ transplant recipients were instructed to use single-handed sampling of each 50 muL capillary blood and dried blood spots by finger prick using standard finger prick devices. We used standardized EDTA-coated capillary blood collection systems and standardized filter paper WS 903. CsA was determined by LC-MS/MS. The patients and technicians also answered a questionnaire on the procedure and sample quality. Results. The C(0) and C(2) levels from capillary blood collection systems (C(0) [ng/mL]: 114.5 +/- 44.5; C(2): 578.2 +/- 222.2) and capil...Continue Reading

References

Jul 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Uta CeglarekJoachim Thiery
Nov 5, 2005·Pediatric Transplantation·Nicholas J A WebbBrian G Keevil
May 9, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·J B BarnardN Yonan
May 30, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·S SchubertF Berger
Jun 27, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Margareta HolubOlaf A Bodamer
Jun 30, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Uta CeglarekJoachim Thiery
Jan 24, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maciej J BoguszMohammed Al Tufail

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Citations

Jun 28, 2011·Analytical and Bioanalytical Chemistry·Julien DéglonChristian Staub
Sep 25, 2014·Mass Spectrometry Reviews·Michel WagnerGérard Hopfgartner
Nov 22, 2014·Pediatric Transplantation·Jane A DickersonRhona M Jack
Feb 8, 2019·European Journal of Clinical Pharmacology·Christel C L M BoonsN Harry Hendrikse
Aug 14, 2013·Annals of Clinical Biochemistry·Edward HinchliffeBrian G Keevil

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