Potential of Electrospun Poly(3-hydroxybutyrate)/Collagen Blends for Tissue Engineering Applications

Journal of Healthcare Engineering
Luca SalvatoreMarta Madaghiele

Abstract

In this work, tunable nonwoven mats based on poly(3-hydroxybutyrate) (PHB) and type I collagen (Coll) were successfully produced by electrospinning. The PHB/Coll weight ratio (fixed at 100/0, 70/30, and 50/50, resp.) was found to control the morphological, thermal, mechanical, and degradation properties of the mats. Increasing collagen amounts led to larger diameters of the fibers (in the approximate range 600-900 nm), while delaying their thermal decomposition (from 245°C to 262°C). Collagen also accelerated the hydrolytic degradation of the mats upon incubation in aqueous medium at 37°C for 23 days (with final weight losses of 1%, 15%, and 23% for 100/0, 70/30, and 50/50 samples, resp.), as a result of increased mat wettability and reduced PHB crystallinity. Interestingly, 70/30 meshes were the ones displaying the lowest stiffness (~116 MPa; p < 0.05 versus 100/0 and 50/50 meshes), while 50/50 samples had an elastic modulus comparable to that of 100/0 ones (~250 MPa), likely due to enhanced physical crosslinking of the collagen chains, at least at high protein amounts. All substrates were also found to allow for good viability and proliferation of murine fibroblasts, up to 6 days of culture. Collectively, the results evidence...Continue Reading

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Citations

Sep 26, 2020·Journal of Functional Biomaterials·Bahareh AzimiSerena Danti
Dec 13, 2018·Journal of Healthcare Engineering·Paola NittiFrancesca Gervaso
Nov 11, 2020·Artificial Organs·Isabella C P RodriguesLaís P Gabriel
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Sevakumaran VigneswariSeeram Ramakrishna
Feb 23, 2021·Frontiers in Bioengineering and Biotechnology·Paola NittiChristian Demitri

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Methods Mentioned

BETA
scanning electron microscopy
atomic force microscopy
differential scanning calorimetry
Assay
AFM

Software Mentioned

ImageJ

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