Potential of Lentibacillus sp. NS12IITR for production of lipids with enriched branched-chain fatty acids for improving biodiesel properties along with hydrocarbon co-production

Extremophiles : Life Under Extreme Conditions
Noopur Singh, Bijan Choudhury

Abstract

Hypersaline environment is inhabited by array of microbes which have the potential to produce industrially important products. This study explored biomass and lipid production potential of the halophilic bacterium, strain NS12IITR which was isolated from Sambhar Lake, Rajasthan. Sequencing and phylogenetic analysis revealed that the bacterium belonged to genus Lentibacillus. The salient feature of the isolate is its ability to accumulate total cellular lipid up to 18.9 ± 0.45% of dry cell weight. In addition, trans-esterification of extracted lipid yielded 77.6 ± 5.56% of total esters as methyl ester of branched-chain fatty acids (BCFAs). To assess the nature of extracted lipid, lipid sample was fractionated on the silicic acid column, which demonstrated that 49.03 ± 1.35% of the total lipids was neutral in nature. Trans-esterification of the neutral lipid fraction yielded 60.62 ± 4.88% of total esters as methyl ester of BCFAs. Methyl esters of BCFAs were present in trans-esterified products of neutral as well as polar lipid fractions. Furthermore, the isolate produced hydrocarbons both extracellularly (C10-C30) and intra-cellularly (C15-C28). The concentration of extracellular hydrocarbon (21.11 ± 0.78 mg/L) synthesized by str...Continue Reading

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Citations

Feb 13, 2021·Frontiers in Bioengineering and Biotechnology·Yilan LiuRadhakrishnan Mahadevan

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Datasets Mentioned

BETA
MF407349

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

MegaX
Basic Local Alignment Search Tool ( BLAST )

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