Potential of Polygonum cuspidatum Root as an Antidiabetic Food: Dual High-Resolution α-Glucosidase and PTP1B Inhibition Profiling Combined with HPLC-HRMS and NMR for Identification of Antidiabetic Constituents

Journal of Agricultural and Food Chemistry
Yong ZhaoDan Staerk

Abstract

The worldwide increasing incidence of type 2 diabetes has fueled an intensified search for food and herbal remedies with preventive and/or therapeutic properties. Polygonum cuspidatum Siebold & Zucc. (Polygonaceae) is used as a functional food in Japan and South Korea, and it is also a well-known traditional antidiabetic herb used in China. In this study, dual high-resolution α-glucosidase and protein-tyrosine phosphatase 1B (PTP1B) inhibition profiling was used for the identification of individual antidiabetic constituents directly from the crude ethyl acetate extract and fractions of P. cuspidatum. Subsequent preparative-scale HPLC was used to isolate a series of α-glucosidase inhibitors, which after HPLC-HRMS and NMR analysis were identified as procyanidin B2 3,3″-O-digallate (3) and (-)-epicatechin gallate (5) with IC50 values of 0.42 ± 0.02 and 0.48 ± 0.0004 μM, respectively, as well as a series of stilbene analogues with IC50 value in the range from 6.05 ± 0.05 to 116.10 ± 2.04 μM. In addition, (trans)-emodin-physcion bianthrone (15b) and (cis)-emodin-physcion bianthrone (15c) were identified as potent PTP1B inhibitors with IC50 values of 2.77 ± 1.23 and 7.29 ± 2.32 μM, respectively. These findings show that P. cuspidatum...Continue Reading

References

Feb 8, 2002·The New England Journal of Medicine·William C KnowlerUNKNOWN Diabetes Prevention Program Research Group
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Jun 26, 2004·Journal of Natural Products·Mamoru OkasakaOzodbek Ashurmetov
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Jan 24, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Hua WeiSong Yang
Nov 7, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Huan ZhangShun-Wan Chan
Apr 22, 2015·Journal of Food Science and Technology·Christian Izuchukwu AbuajahChijioke Maduka Osuji

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Citations

Oct 3, 2019·Biomolecules·Bahare SalehiJavad Sharifi-Rad
Nov 28, 2019·Phytotherapy Research : PTR·Aminu MohammedMurtala Bindawa Isah
Jan 19, 2021·Journal of Asian Natural Products Research·Xu ZhangPei-Cheng Zhang
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin LiZongsuo Liang
Jun 1, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Min WangRui Song

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