Potential of vancomycin for the enantiomeric resolution of FMOC-amino acids by capillary electrophoresis-ion-trap-mass spectrometry

Electrophoresis
Laura Sánchez-HernándezAntonio L Crego

Abstract

The potential of the antibiotic vancomycin (VC) as chiral selector for the enantiomeric separation of amino acids by CE-ESI-MS/MS² was investigated for the first time in this work. Derivatization of amino acids with FMOC-Cl was carried out to enable their interaction with VC as well as the formation of precursor ions with larger m/z which were employed in MS² experiments. The partial filling of a coated capillary was employed to avoid the loss in MS sensitivity originated by the introduction of VC in the ionization source. Under optimized conditions, the simultaneous enantiomeric separation and unequivocal identification of 17 amino acids (two of them being nonprotein amino acids) took place in about 20 min with LODs in the micromolar range.

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Citations

Oct 6, 2015·Electrophoresis·Véréna PoinsotFrançois Couderc
Oct 6, 2015·Electrophoresis·Elena Sánchez-LópezAntonio L Crego
Sep 30, 2014·Electrophoresis·Ioannis J StavrouConstantina P Kapnissi-Christodoulou
Jul 4, 2016·Journal of Chromatography. a·Raquel Pérez-MíguezMaría Castro-Puyana
Jul 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Éva SzökőTamás Tábi
Oct 17, 2015·Electrophoresis·Elena Domínguez-VegaMaria Luisa Marina
Aug 11, 2015·Electrophoresis·Alain Wuethrich, Joselito P Quirino
Jun 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katarzyna PauterBogusław Buszewski

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