Potential pathogens, antimicrobial patterns and genotypic diversity of Escherichia coli isolates in constructed wetlands treating swine wastewater

FEMS Microbiology Ecology
A Mark IbekweGudigopura B Reddy

Abstract

Escherichia coli populations originating from swine houses through constructed wetlands were analyzed for potential pathogens, antimicrobial susceptibility patterns, and genotypic diversity. Escherichia coli isolates (n = 493) were screened for the presence of the following virulence genes: stx1, stx2 and eae (Shiga toxin-producing E. coli [STEC]), heat-labile enterotoxin (LT) genes and heat stable toxin STa and STb (enterotoxigenic E. coli (ETEC), cytotoxin necrotizing factors 1 and 2 (cnf1 and cnf2 [necrotoxigenic E. coli- NTEC]), as well as O and H antigens, and the presence of the antibiotic resistance genes blaTEM, blaSHV, blaCMY-2, tet A, tet B, tet C, mph(A), aadA, StrA/B, sul1, sul2 and sul3. The commensal strains were further screened for 16 antimicrobials and characterized by BOX AIR-1 PCR for unique genotypes. The highest antibiotic resistance prevalence was for tetracycline, followed by erythromycin, ampicillin, streptomycin, sulfisoxazole and kanamycin. Our data showed that most of the isolates had high distribution of single or multidrug-resistant (MDR) genotypes. Therefore, the occurrence of MDR E. coli in the wetland is a matter of great concern due to possible transfer of resistance genes from nonpathogenic to ...Continue Reading

References

Jul 17, 1998·Clinical Microbiology Reviews·J C Paton, A W Paton
May 3, 2000·Journal of Dairy Science·J B RussellG N Jarvis
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·David L SmithJ Glenn Morris
Aug 15, 2002·Journal of Environmental Quality·Alexandria K GravesRaymond B Reneau
Aug 23, 2002·Journal of Internal Medicine·B Henriques Normark, S Normark
Mar 26, 2003·Journal of Microbiological Methods·Douglas R CallFrank J Loge
Sep 6, 2003·Applied and Environmental Microbiology·A Mark IbekweStephen R Lyon
Mar 6, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Alicia D AndersonFrederick J Angulo
Dec 22, 2005·Foodborne Pathogens and Disease·Shelton E MurindaStephen P Oliver
Jan 21, 2006·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Michael J CasteelJ Paul Mueller
Jul 6, 2006·Applied and Environmental Microbiology·Toni A ChapmanJames J-C Chin
Dec 22, 2006·Environmental Science & Technology·Amy PrudenKenneth H Carlson
Jun 22, 2007·Water Research·A K GravesE Martin
Nov 23, 2007·The Science of the Total Environment·Richard L WhitmanMuruleedhara N Byappanahalli
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Jan 31, 2009·Chemosphere·Klaus Kümmerer
Sep 26, 2009·Journal of Applied Microbiology·A K Graves, R W Weaver
Oct 27, 2009·The Science of the Total Environment·Pierre Servais, Julien Passerat
Oct 29, 2009·Emerging Infectious Diseases·Minh Chau Phuc NguyenAnnie Canu
Apr 20, 2010·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Dean A ForbesJohnsely S Cyrus
Nov 11, 2010·Analytical and Bioanalytical Chemistry·Despo Fatta-KassinosAnastasia Nikolaou
Jan 18, 2011·Applied and Environmental Microbiology·Dayna M Brichta-HarhayMohammad Koohmaraie
Apr 26, 2011·Applied and Environmental Microbiology·Dustin K Goto, Tao Yan
Jan 1, 2008·Microbes and Environments·Satoshi Ishii, Michael J Sadowsky
Nov 10, 2011·FEMS Microbiology Ecology·Abasiofiok Mark IbekweAlexandria K Graves
Oct 6, 2012·Environmental Science & Technology·Amy PrudenHeather N Storteboom
Nov 20, 2012·Applied and Environmental Microbiology·Dominic FrigonRonald Gehr
May 30, 2013·The Science of the Total Environment·Anabela PereiraAntónio Correia
Mar 31, 2015·Nature Reviews. Microbiology·Thomas U BerendonkJosé Luis Martinez

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