Potential protection of green tea polyphenols against ultraviolet irradiation-induced injury on rat cortical neurons

Neuroscience Letters
Mei-Li Liu, Long-Chuan Yu

Abstract

The present study was performed to investigate the possible protective effects of green tea polyphenols against ultraviolet (UV)-C light irradiation-induced cell death in the cultured rat cortical neurons. We found that UV-C light irradiation induced marked cell death tested by 3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and TdT-mediated biotin-dUTP nicked-end labeling (TUNEL) assay. Protective effects of green tea polyphenols on UV-C light irradiation-induced apoptosis in cortical neurons were demonstrated by testing the content of Bax, which is involved in cell death. The expression of active Bax in cultured rat cortical neurons was inhibited significantly by green tea polyphenols compared to UV irradiation group tested by the immunoprecipitation assay and Western blot assay. However, there were no significant changes in the contents of total Bax after treatment with green tea polyphenols in UV-C light-irradiated rat cortical neurons. Our results demonstrated that the green tea polyphenols inhibited the active Bax expression, suggesting a neuroprotective effect of green tea polyphenols against the UV-C light irradiation-induced injury on cortical neurons.

References

Oct 3, 1998·Journal of Photochemistry and Photobiology. B, Biology·T Schwarz
Oct 31, 1998·Nutrition and Cancer·J L Bushman
Jun 30, 2000·Current Opinion in Cell Biology·M Crompton
Mar 30, 2001·Cell Death and Differentiation·R S Morrison, Y Kinoshita
May 16, 2003·Journal of Neuroscience Research·Sriranganathan VarathanTakashi Mashimo
Dec 13, 2005·Expert Review of Anticancer Therapy·Melissa M CamouseElma D Baron
Oct 19, 2006·Journal of the National Cancer Institute·Arata TomiyamaChifumi Kitanaka
May 4, 2007·Journal of Virology·Dana WestphalAndrew A Mercer

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Citations

May 22, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Alla I PotapovichLiudmila G Korkina
Jan 22, 2010·Journal of Neuroscience Research·XiaoJing LinLong-Chuan Yu
Nov 20, 2016·Journal of Tissue Engineering and Regenerative Medicine·Meili LiuYubo Fan

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis