Potential role of β-elemene on histone H1 in the H22 ascites hepatoma cell line

Molecular Medicine Reports
Fazhen BaoHong Zhang

Abstract

β-Elemene is extracted from the Chinese medicinal herb Curcuma Wenyujin. It has a broad-spectrum antitumor effect on many types of cancer. However, the exact mechanism of action of β-elemene in hepatocellular carcinoma (HCC) remains unknown. Histone H1 is thought to act as a repressor of transcription by promoting the compaction of chromatin into higher order structures. We found that histone H1 plays an important role in the antitumor function of β-elemene. In this study, histone H1 expression in the H22 murine hepatocellular carcinoma cell line was confirmed, with P388D1 cells serving as a positive control. Furthermore, H22 cells were cultured with β-elemene for different time-points in vitro and treated with different dose-dependent β-elemene in an experimental H22 HCC xenograft transplantation model to confirm whether β-elemene inhibited the growth of H22 tumor cells. In addition, measurements of histone H1 expression both in vitro and in vivo enabled us to demonstrate that β-elemene affects the expression of histone H1 only at the protein level, but is not involved in regulation at the gene level. Our results clearly show that the effect of β-elemene on inhibiting the growth of H22 tumor cells is time- and dose-dependent. ...Continue Reading

Citations

Feb 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bing HuLing Xu
Apr 9, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Yang ZhouGaimei She
May 17, 2014·Phytotherapy Research : PTR·Jean-François LesgardsSylvia Pietri
Jul 13, 2013·Molecular Nutrition & Food Research·Bharat B AggarwalSubash C Gupta
Sep 15, 2020·Biomicrofluidics·Zhengcheng LuDayou Li

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