Potential role of 20S proteasome in maintaining stem cell integrity of human bone marrow stromal cells in prolonged culture expansion

Biochemical and Biophysical Research Communications
Li LuZhi-Cheng Xiao

Abstract

Human bone marrow stromal cells (hBMSCs) could be used in clinics as precursors of multiple cell lineages following proper induction. Such application is impeded by their characteristically short lifespan, together with the increasing loss of proliferation capability and progressive reduction of differentiation potential after the prolonged culture expansion. In the current study, we addressed the possible role of 20S proteasomes in this process. Consistent with prior reports, long-term in vitro expansion of hBMSCs decreased cell proliferation and increased replicative senescence, accompanied by reduced activity and expression of the catalytic subunits PSMB5 and PSMB1, and the 20S proteasome overall. Application of the proteasome inhibitor MG132 produced a senescence-like phenotype in early passages, whereas treating late-passage cells with 18α-glycyrrhetinic acid (18α-GA), an agonist of 20S proteasomes, delayed the senescence progress, enhancing the proliferation and recovering the capability of differentiation. The data demonstrate that activation of 20S proteasomes assists in counteracting replicative senescence of hBMSCs expanded in vitro.

References

Jun 19, 1981·European Journal of Pharmacology·J van MarleJ van Weeren-Kramer
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Oct 29, 2000·Experimental Gerontology·A L BulteauB Friguet
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J O'HareP S Jat
Apr 20, 2001·Mechanisms of Ageing and Development·M GaczynskaW F Ward
May 9, 2001·Biochemical and Biophysical Research Communications·H J TaylorT M Griffith
Jan 22, 2004·Archives of Medical Research·Brenton ShortPaul J Simmons
Jun 21, 2005·Molecular & Cellular Proteomics : MCP·Julia R HayterRobert J Beynon
Oct 26, 2005·Experimental Gerontology·Niki Chondrogianni, Efstathios S Gonos
Apr 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vita A VernaceMaria E Figueiredo-Pereira
Jan 14, 2010·The Journal of Biological Chemistry·Suzanne KapetaEfstathios S Gonos
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yuki KujuroToru Kondo
May 17, 2011·BMC Medicine·Alan TrounsonDon Gibbons

❮ Previous
Next ❯

Citations

Jul 24, 2014·Cellular and Molecular Life Sciences : CMLS·Eun Seong Hwang
Jan 8, 2014·Biochemical and Biophysical Research Communications·Li LuWu-Lin Yang
May 17, 2014·Molecular & Cellular Proteomics : MCP·Bamaprasad DuttaSiu Kwan Sze

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.