Potential Role of Long Non-Coding RNA ANRIL in Pediatric Medulloblastoma Through Promotion on Proliferation and Migration by Targeting miR-323.

Journal of Cellular Biochemistry
Hong ZhangXinxin Chen

Abstract

Medulloblastoma (MB) is a common primary tumor of the central nervous system diagnosed in pediatric patients. Survivors of MB are frequently accompanied by severe side effects, thus it is urgent to explore novel therapeutic target. First of all, the level of antisense non-coding RNA in the INK4 locus (ANRIL) in normal cerebellum and DAOY cells were evaluated. Then, the effect of ANRIL on DAOY cell proliferation, migration, and apoptosis were tested. Then, qRT-PCR was performed to explore whether ANRIL acted as a sponge of miR-323. The effects of miR-323 inhibition on ANRIL silence-induced alterations of cell proliferation, migration, and apoptosis were estimated. Predicted by TargetScan, the possible target gene of miR-323 was screened, followed by validation with luciferase assay. The abnormally expressed BRI3 on cell proliferation, migration, apoptosis, and signaling pathways were all evaluated. ANRIL was up-regulated in MB and its silence significantly lowered cell viability and migration but promoted cell apoptosis. ANRIL acted as a sponge of miR-323 and its silence functioned through up-regulating miR-323. BRI3 and CDK6 were target genes of miR-323 and the effect of BRI3 on DAOY cells was the same as ANRIL. Moreover, ANRIL...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
Nov 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Frank MendrzykPeter Lichter
Oct 16, 2009·The New England Journal of Medicine·Richard S HotchkissPaul E Swanson
Nov 3, 2010·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Aug 28, 2014·Biochimica Et Biophysica Acta·Guodong YangLijun Yuan
Sep 3, 2014·The Journal of Clinical Investigation·Fabien LoisonHongbo R Luo
Sep 4, 2015·Journal of Child Neurology·Nathan E Millard, Kevin C De Braganca
Mar 16, 2016·International Journal of Molecular Sciences·Ahmed S BayoumiIl-Man Kim
Mar 30, 2016·Current Neurology and Neuroscience Reports·Daniel ColucciaJames T Rutka
Jul 14, 2016·Journal of Neuro-oncology·Yongxiao LiQing Chang

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Citations

Aug 22, 2020·Cancer Biotherapy & Radiopharmaceuticals·Yufeng GaoDongnan Hou
Sep 29, 2020·Future Oncology·Ning LouYunbao Pan
Mar 30, 2019·Frontiers in Pediatrics·Pietro LaneveElisa Caffarelli
Feb 6, 2020·Clinical and Translational Medicine·Estanislao NavarroMiguel Hueso
May 10, 2019·Journal of Cellular Physiology·Golamreza JadideslamAlireza Kabbazi
Jun 13, 2020·Frontiers in Cell and Developmental Biology·Pietro Laneve, Elisa Caffarelli
May 16, 2018·Cancer Biomarkers : Section a of Disease Markers·Bing ChenFeng Xu
Jun 28, 2020·BMC Medical Genomics·Varun KesherwaniNagendra K Chaturvedi
Aug 9, 2018·Frontiers in Endocrinology·Yahui KongLaura C Alonso
Jun 3, 2021·International Journal of Molecular Sciences·Agata StrejczekAnna-Maria Barciszewska
Jul 29, 2021·NAR Cancer·Keisuke KatsushimaRanjan J Perera

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